| Literature DB >> 18329166 |
Akihiko Watanabe1, Remon Otake, Tetsuo Nozaki, Akihiro Morii, Ryohei Ogawa, Shinichi Fujimoto, Shinobu Nakamura, Hideki Fuse, Takashi Kondo.
Abstract
We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1MHz ultrasound at 0.2MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454>MRX-815H>Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.Entities:
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Year: 2008 PMID： 18329166 DOI： 10.1016/j.canlet.2008.02.004
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679