| Literature DB >> 18328559 |
Ruriko Tanaka1, Junya Kuroda, William Stevenson, Eishi Ashihara, Takayuki Ishikawa, Tomohiko Taki, Yutaka Kobayashi, Yuri Kamitsuji, Eri Kawata, Miki Takeuchi, Yoshihide Murotani, Asumi Yokota, Mitsuharu Hirai, Satoshi Majima, Masafumi Taniwaki, Taira Maekawa, Shinya Kimura.
Abstract
JAK2V617F is a common mutation in chronic myeloproliferative diseases (CMPDs). We have developed a system utilizing JAK2V617F-specific guanine quenching probe (QP-system) to detect JAK2V617F. With QP-system, results can be obtained from 100 microl of blood within 90 min. We compared QP-system with direct sequencing using 42 CMPD patients' specimens. JAK2V617F was detected in 25 specimens by QP-system, while direct sequencing failed to detect JAK2V617F in 7 of those 25. The presence of JAK2V617F mutation in these 7 specimens was confirmed by allele-specific PCR. These findings indicate that QP-system is more sensitive and useful than direct sequencing for diagnoses of CMPDs.Entities:
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Year: 2008 PMID： 18328559 DOI： 10.1016/j.leukres.2007.12.019
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156