Literature DB >> 1832732

Effect of 4,7,10,13,16,19-docosahexaenoic acid on triglyceride accumulation and secretion in rat hepatocytes in culture.

L J Martin1, G B Reaidi, G R Gavino, V C Gavino.   

Abstract

We have investigated the effect of oleic (18:1) and 4,7,10,13,16,19-docosahexaenoic (22:6 omega 3) acids on triglyceride (TG) accumulation, secretion and reuptake in rat hepatocytes in culture. We also calculated the percentage of total TG, TG-esterified 18:1 and TG-esterified 22:6 omega 3 that were secreted relative to the total accumulation (intra + extracellular TG). Both fatty acids were incorporated mainly in the intracellular TG fraction. Treatment with 18:1 but not with 22:6 omega 3 increased the quantity of TG secreted into the culture medium relative to controls. Treatment with 22:6 omega 3 caused a greater accumulation of intracellular TG than 18:1. This arises in part from the preferential retention of 22:6 omega 3-enriched TG by the hepatocytes. At 24 hr, there was no longer any difference in the net secretion of TG by 18:1 and 22:6 omega 3-treated cells, which may be a consequence of the reuptake of TG-esterified 18:1. There was no reuptake of TG-esterified 22:6 omega 3. We conclude that inhibition of hepatocyte TG synthesis is not obligatory for 22:6 omega 3-induced diminution of TG secretion.

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Year:  1991        PMID： 1832732     DOI： 10.1007/bf02537202

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  27 in total

1.  The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes.

Authors:  Z M Yao; D E Vance
Journal:  J Biol Chem       Date:  1988-02-25       Impact factor: 5.157

Review 2.  Fatty acid binding to plasma albumin.

Authors:  A A Spector
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3.  Eicosapentaenoic acid inhibits synthesis and secretion of triacylglycerols by cultured rat hepatocytes.

Authors:  J O Nossen; A C Rustan; S H Gloppestad; S Målbakken; C A Drevon
Journal:  Biochim Biophys Acta       Date:  1986-10-24

4.  Inhibition of apolipoprotein secretion and phosphatidate phosphohydrolase activity by eicosapentaenoic and docosahexaenoic acids in the perfused rat liver.

Authors:  S H Wong; J B Marsh
Journal:  Metabolism       Date:  1988-12       Impact factor: 8.694

5.  Effects of eicosapentaenoic and docosahexaenoic acids on apoprotein B mRNA and secretion of very low density lipoprotein in HepG2 cells.

Authors:  S H Wong; E A Fisher; J B Marsh
Journal:  Arteriosclerosis       Date:  1989 Nov-Dec

6.  Hepatic fatty acid synthesis and triglyceride secretion in rats fed fructose- or glucose-based diets containing corn oil, tallow or marine oil.

Authors:  G R Herzberg; M Rogerson
Journal:  J Nutr       Date:  1988-09       Impact factor: 4.798

7.  Contribution of cytoplasmic storage triacylglycerol to VLDL-triacylglycerol in isolated rat hepatocytes.

Authors:  O L Francone; A D Kalopissis; G Griffaton
Journal:  Biochim Biophys Acta       Date:  1989-03-14

8.  Secretion and storage of newly synthesized hepatic triacylglycerol fatty acids in vivo in different nutritional states and in diabetes.

Authors:  J M Duerden; G F Gibbons
Journal:  Biochem J       Date:  1988-11-01       Impact factor: 3.857

9.  Polyunsaturated fatty acid accumulation in the lipids of cultured fibroblasts and smooth muscle cells.

Authors:  V C Gavino; J S Miller; J M Dillman; G E Milo; D G Cornwell
Journal:  J Lipid Res       Date:  1981-01       Impact factor: 5.922

10.  Alpha-tocopherol and inhibition of cytolysis in glutathione-depleted hepatocytes in primary culture.

Authors:  I Hishinuma; T Nakamura
Journal:  J Nutr Sci Vitaminol (Tokyo)       Date:  1988-02       Impact factor: 2.000
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  7 in total

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Journal:  Lipids       Date:  2005-05       Impact factor: 1.880

2.  Eicosapentaenoic acid, but not docosahexaenoic acid, increases mitochondrial fatty acid oxidation and upregulates 2,4-dienoyl-CoA reductase gene expression in rats.

Authors:  N Willumsen; H Vaagenes; O Lie; A C Rustan; R K Berge
Journal:  Lipids       Date:  1996-06       Impact factor: 1.880

3.  Eicosapentaenoic acid is primarily responsible for hypotriglyceridemic effect of fish oil in humans.

Authors:  G S Rambjør; A I Wålen; S L Windsor; W S Harris
Journal:  Lipids       Date:  1996-03       Impact factor: 1.880

4.  Effects of highly purified eicosapentaenoic acid and docosahexaenoic acid on fatty acid absorption, incorporation into serum phospholipids and postprandial triglyceridemia.

Authors:  J B Hansen; S Grimsgaard; H Nilsen; A Nordøy; K H Bønaa
Journal:  Lipids       Date:  1998-02       Impact factor: 1.880

5.  Eicosapentaenoic acid inhibits cell growth and triacylglycerol secretion in McA-RH7777 rat hepatoma cultures.

Authors:  J C Fox; R V Hay
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

6.  Chronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs.

Authors:  L Frøyland; H Vaagenes; D K Asiedu; A Garras; O Lie; R K Berge
Journal:  Lipids       Date:  1996-02       Impact factor: 1.880

7.  Bioconversion of α-linolenic acid into n-3 long-chain polyunsaturated fatty acid in hepatocytes and ad hoc cell culture optimisation.

Authors:  Ramez Alhazzaa; Andrew J Sinclair; Giovanni M Turchini
Journal:  PLoS One       Date:  2013-09-11       Impact factor: 3.240
  7 in total

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