OBJECTIVE: To study a method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel and develop an assay correspondingly. METHODS: After extracting the freeze dried gel with acetic ether, detected it in colorimetric method. RESULTS: The interference of poloxamer was removed thoroughly, while the retention rate of the polysacchrarides showed to be 100.11% (n = 3). The average recovery was 102.6% (n = 6). CONCLUSION: The method shows to be simple, maneuverable, stable and well reproducible.
OBJECTIVE: To study a method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel and develop an assay correspondingly. METHODS: After extracting the freeze dried gel with acetic ether, detected it in colorimetric method. RESULTS: The interference of poloxamer was removed thoroughly, while the retention rate of the polysacchrarides showed to be 100.11% (n = 3). The average recovery was 102.6% (n = 6). CONCLUSION: The method shows to be simple, maneuverable, stable and well reproducible.