Literature DB >> 18322070

Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds.

Zheng Xing1, Carol Cardona, Phuong Dao, Beate Crossley, Sharon Hietala, Walter Boyce.   

Abstract

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.

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Year:  2008        PMID： 18322070      PMCID： PMC2395069          DOI： 10.1128/JCM.02426-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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