Literature DB >> 1831977

A human Fab fragment specific for thyroid peroxidase generated by cloning thyroid lymphocyte-derived immunoglobulin genes in a bacteriophage lambda library.

S Portolano1, P Seto, G D Chazenbalk, Y Nagayama, S M McLachlan, B Rapoport.   

Abstract

A human Fab fragment (SP2) which binds specifically to human thyroid peroxidase has been generated by expressing random combinations of heavy and light chain immunoglobulin genes (derived from Graves' thyroid cDNA) in a bacteriophage lambda library. In common with many serum TPO autoantibodies, the cloned Fab fragment is IgG1 kappa and has a high affinity for TPO (approximately 10(-9) M). On the basis of their nucleotide sequences, the heavy and light chain genes coding for SP2 belong to families VHI, (D), JH3 and VKI, JK2, respectively. These data provide the first characterization at a molecular level of a human thyroid peroxidase antibody associated with autoimmune thyroid disease.

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Year:  1991        PMID： 1831977     DOI： 10.1016/0006-291x(91)91380-u

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Thyroid autoimmune disease: demonstration of thyroid antigen-specific B cells and recombination-activating gene expression in chemokine-containing active intrathyroidal germinal centers.

Authors:  M P Armengol; M Juan; A Lucas-Martín; M T Fernández-Figueras; D Jaraquemada; T Gallart; R Pujol-Borrell
Journal:  Am J Pathol       Date:  2001-09       Impact factor: 4.307

2.  Relationship between autoantibody epitopic recognition and immunoglobulin gene usage.

Authors:  J Guo; R S Mcintosh; B Czarnocka; A P Weetman; B Rapoport; S M McLachlan
Journal:  Clin Exp Immunol       Date:  1998-02       Impact factor: 4.330

Review 3.  Immunity to the thyroid-stimulating hormone receptor.

Authors:  J Furmaniak; B R Smith
Journal:  Springer Semin Immunopathol       Date:  1993

4.  Human organ-specific autoimmune disease. Molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic immunodominant region and restricted immunoglobulin gene usage in the target organ.

Authors:  G D Chazenbalk; S Portolano; D Russo; J S Hutchison; B Rapoport; S McLachlan
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

Review 5.  Genetic and epitopic analysis of thyroid peroxidase (TPO) autoantibodies: markers of the human thyroid autoimmune response.

Authors:  S M McLachlan; B Rapoport
Journal:  Clin Exp Immunol       Date:  1995-08       Impact factor: 4.330

6.  Recognition by recombinant autoimmune thyroid disease-derived Fab fragments of a dominant conformational epitope on human thyroid peroxidase.

Authors:  S Portolano; G D Chazenbalk; P Seto; J S Hutchison; B Rapoport; S M McLachlan
Journal:  J Clin Invest       Date:  1992-09       Impact factor: 14.808

7.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971
  7 in total

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