OBJECTIVE: Genome-wide scans were conducted in search for genetic locations linked to energy expenditure and substrate oxidation in children. DESIGN: Pedigreed data of 1030 Hispanic children and adolescents were from the Viva La Familia Study which was designed to investigate genetic and environmental risk factors for the development of obesity in Hispanic families. A respiratory calorimeter was used to measure 24-h total energy expenditure (TEE), basal metabolic rate (BMR), sleep metabolic rate (SMR), 24-h respiratory quotient (24RQ), basal metabolic respiratory quotient (BMRQ) and sleep respiratory quotient (SRQ). Protein, fat and carbohydrate oxidation (PROOX, FATOX and CHOOX, respectively) were also estimated. All participants were genotyped for 384 single tandem repeat markers spaced an average of 10 cM apart. Computer program SOLAR was used to perform the genetic linkage analyses. RESULTS: Significant linkage for TEE was detected on chromosome 1 near marker D1S2841, with a logarithm of the odds (LOD) score of 4.0. SMR, BMRQ and PROOX were associated with loci on chromosome 18, 17 and 9, respectively, with LOD scores of 4.88, 3.17 and 4.55, respectively. A genome-wide scan of SMR per kg fat-free mass (SpFFM) peaked in the same region as SMR on chromosome 18 (LOD, 5.24). Suggestive linkage was observed for CHOOX and FATOX. Several candidate genes were found in the above chromosomal regions including leptin receptor (LEPR). CONCLUSION: Regions on chromosomes 1, 9, 17 and 18 harbor genes affecting variation in energy expenditure and substrate oxidation in Hispanic children and adolescents.
OBJECTIVE: Genome-wide scans were conducted in search for genetic locations linked to energy expenditure and substrate oxidation in children. DESIGN: Pedigreed data of 1030 Hispanic children and adolescents were from the Viva La Familia Study which was designed to investigate genetic and environmental risk factors for the development of obesity in Hispanic families. A respiratory calorimeter was used to measure 24-h total energy expenditure (TEE), basal metabolic rate (BMR), sleep metabolic rate (SMR), 24-h respiratory quotient (24RQ), basal metabolic respiratory quotient (BMRQ) and sleep respiratory quotient (SRQ). Protein, fat and carbohydrate oxidation (PROOX, FATOX and CHOOX, respectively) were also estimated. All participants were genotyped for 384 single tandem repeat markers spaced an average of 10 cM apart. Computer program SOLAR was used to perform the genetic linkage analyses. RESULTS: Significant linkage for TEE was detected on chromosome 1 near marker D1S2841, with a logarithm of the odds (LOD) score of 4.0. SMR, BMRQ and PROOX were associated with loci on chromosome 18, 17 and 9, respectively, with LOD scores of 4.88, 3.17 and 4.55, respectively. A genome-wide scan of SMR per kg fat-free mass (SpFFM) peaked in the same region as SMR on chromosome 18 (LOD, 5.24). Suggestive linkage was observed for CHOOX and FATOX. Several candidate genes were found in the above chromosomal regions including leptin receptor (LEPR). CONCLUSION: Regions on chromosomes 1, 9, 17 and 18 harbor genes affecting variation in energy expenditure and substrate oxidation in Hispanic children and adolescents.
Authors: Nancy F Butte; Yan Liu; Issa F Zakeri; Robert P Mohney; Nitesh Mehta; V Saroja Voruganti; Harald Göring; Shelley A Cole; Anthony G Comuzzie Journal: Am J Clin Nutr Date: 2015-06-17 Impact factor: 7.045
Authors: Pei-An Betty Shih; Lei Wang; Stephane Chiron; Gen Wen; Caroline Nievergelt; Manjula Mahata; Srikrishna Khandrika; Fangwen Rao; Maple M Fung; Sushil K Mahata; Bruce A Hamilton; Daniel T O'Connor Journal: J Clin Endocrinol Metab Date: 2009-10-09 Impact factor: 5.958
Authors: Paolo Piaggi; Çiğdem Köroğlu; Anup K Nair; Jeff Sutherland; Yunhua L Muller; Pankaj Kumar; Wen-Chi Hsueh; Sayuko Kobes; Alan R Shuldiner; Hye In Kim; Nehal Gosalia; Cristopher V Van Hout; Marcus Jones; William C Knowler; Jonathan Krakoff; Robert L Hanson; Clifton Bogardus; Leslie J Baier Journal: J Clin Endocrinol Metab Date: 2020-11-01 Impact factor: 5.958
Authors: Anthony G Comuzzie; Shelley A Cole; Sandra L Laston; V Saroja Voruganti; Karin Haack; Richard A Gibbs; Nancy F Butte Journal: PLoS One Date: 2012-12-14 Impact factor: 3.240