Literature DB >> 18314740

ATP production in isolated mitochondria of procyclic Trypanosoma brucei.

André Schneider1, Nabile Bouzaidi-Tiali, Anne-Laure Chanez, Laurence Bulliard.   

Abstract

This chapter describes a luciferase-based protocol to measure adenosine triphosphate (ATP) production in isolated mitochondria of Trypanosoma brucei. The assay represents an excellent method to characterize the functionality of isolated mitochondria. Comparing the ATP production induced by substrates for oxidative phosphorylation to the one induced by substrates for substrate-level phosphorylation allows conclusions regarding the integrity of the outer and inner mitochondrial membranes. Furthermore, the assay is a valuable tool for characterization of RNA interference cell lines suspected to affect mitochondrial functions.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 18314740     DOI： 10.1007/978-1-59745-365-3_27

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Pentatricopeptide repeat proteins in Trypanosoma brucei function in mitochondrial ribosomes.

Authors:  Mascha Pusnik; Ian Small; Laurie K Read; Thomas Fabbro; André Schneider
Journal:  Mol Cell Biol       Date:  2007-07-23       Impact factor: 4.272

2.  In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.

Authors:  Florence Tschopp; Fabien Charrière; André Schneider
Journal:  EMBO Rep       Date:  2011-07-01       Impact factor: 8.807

3.  Biogenesis of a Mitochondrial Outer Membrane Protein in Trypanosoma brucei: TARGETING SIGNAL AND DEPENDENCE ON A UNIQUE BIOGENESIS FACTOR.

Authors:  Julia Bruggisser; Sandro Käser; Jan Mani; André Schneider
Journal:  J Biol Chem       Date:  2017-01-18       Impact factor: 5.157

4.  Role of Δ1-pyrroline-5-carboxylate dehydrogenase supports mitochondrial metabolism and host-cell invasion of Trypanosoma cruzi.

Authors:  Brian S Mantilla; Lisvane S Paes; Elizabeth M F Pral; Daiana E Martil; Otavio H Thiemann; Patricio Fernández-Silva; Erick L Bastos; Ariel M Silber
Journal:  J Biol Chem       Date:  2015-01-26       Impact factor: 5.157

5.  An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote.

Authors:  Mauro Serricchio; Peter Bütikofer
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-26       Impact factor: 11.205

6.  Functional characterization of TbMCP5, a conserved and essential ADP/ATP carrier present in the mitochondrion of the human pathogen Trypanosoma brucei.

Authors:  Priscila Peña-Diaz; Ludovic Pelosi; Charles Ebikeme; Claudia Colasante; Fei Gao; Frederic Bringaud; Frank Voncken
Journal:  J Biol Chem       Date:  2012-10-16       Impact factor: 5.157

7.  Dual targeting of a tRNAAsp requires two different aspartyl-tRNA synthetases in Trypanosoma brucei.

Authors:  Fabien Charrière; Patrick O'Donoghue; Sunna Helgadóttir; Laurence Maréchal-Drouard; Marina Cristodero; Elke K Horn; Dieter Söll; André Schneider
Journal:  J Biol Chem       Date:  2009-04-22       Impact factor: 5.157

8.  Downregulation of mitochondrial porin inhibits cell growth and alters respiratory phenotype in Trypanosoma brucei.

Authors:  Ujjal K Singha; Shvetank Sharma; Minu Chaudhuri
Journal:  Eukaryot Cell       Date:  2009-07-17

9.  Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1.

Authors:  Megan L Povelones; Calvin Tiengwe; Eva Gluenz; Keith Gull; Paul T Englund; Robert E Jensen
Journal:  Mol Microbiol       Date:  2013-01-21       Impact factor: 3.501

10.  An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids.

Authors:  Mascha Pusnik; Jan Mani; Oliver Schmidt; Moritz Niemann; Silke Oeljeklaus; Felix Schnarwiler; Bettina Warscheid; Trevor Lithgow; Chris Meisinger; André Schneider
Journal:  Mol Biol Cell       Date:  2012-07-11       Impact factor: 4.138
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.