| Literature DB >> 18313904 |
Kosuke Nishio1, Yuka Masaike, Morihito Ikeda, Hiroki Narimatsu, Nobuyuki Gokon, Shingo Tsubouchi, Mamoru Hatakeyama, Satoshi Sakamoto, Naohiro Hanyu, Adarsh Sandhu, Haruma Kawaguchi, Masanori Abe, Hiroshi Handa.
Abstract
We developed novel magnetic nano-carriers around 180 nm in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nano-carriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein.Entities:
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Year: 2008 PMID： 18313904 DOI： 10.1016/j.colsurfb.2008.01.013
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268