Literature DB >> 18313039

Structural analysis of the alpha-D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme.

Sander S van Leeuwen1, Slavko Kralj, Ineke H van Geel-Schutten, Gerrit J Gerwig, Lubbert Dijkhuizen, Johannis P Kamerling.   

Abstract

The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1-->4,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows: [Formula: see text].

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18313039     DOI： 10.1016/j.carres.2008.01.044

Source DB:  PubMed          Journal:  Carbohydr Res        ISSN： 0008-6215            Impact factor:   2.104


  10 in total

1.  Phosphate incorporation during glycogen synthesis and Lafora disease.

Authors:  Vincent S Tagliabracci; Christian Heiss; Chandra Karthik; Christopher J Contreras; John Glushka; Mayumi Ishihara; Parastoo Azadi; Thomas D Hurley; Anna A DePaoli-Roach; Peter J Roach
Journal:  Cell Metab       Date:  2011-03-02       Impact factor: 27.287

2.  Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron.

Authors:  Alicia Lammerts van Bueren; Aakanksha Saraf; Eric C Martens; Lubbert Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  2015-04-03       Impact factor: 4.792

3.  4,6-α-glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families 13 and 70.

Authors:  Slavko Kralj; Pieter Grijpstra; Sander S van Leeuwen; Hans Leemhuis; Justyna M Dobruchowska; Rachel M van der Kaaij; Amarila Malik; Ariyanti Oetari; Johannis P Kamerling; Lubbert Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  2011-09-23       Impact factor: 4.792

4.  4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily.

Authors:  Hans Leemhuis; Willem P Dijkman; Justyna M Dobruchowska; Tjaard Pijning; Pieter Grijpstra; Slavko Kralj; Johannis P Kamerling; Lubbert Dijkhuizen
Journal:  Appl Microbiol Biotechnol       Date:  2012-02-25       Impact factor: 4.813

5.  Structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of Lactobacillus reuteri.

Authors:  Xiangfeng Meng; Tjaard Pijning; Justyna M Dobruchowska; Huifang Yin; Gerrit J Gerwig; Lubbert Dijkhuizen
Journal:  Sci Rep       Date:  2016-10-17       Impact factor: 4.379

Review 6.  Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.

Authors:  Xiangfeng Meng; Joana Gangoiti; Yuxiang Bai; Tjaard Pijning; Sander S Van Leeuwen; Lubbert Dijkhuizen
Journal:  Cell Mol Life Sci       Date:  2016-05-07       Impact factor: 9.261

7.  4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H.

Authors:  Joana Gangoiti; Sander S van Leeuwen; Gerrit J Gerwig; Stéphane Duboux; Christina Vafiadi; Tjaard Pijning; Lubbert Dijkhuizen
Journal:  Sci Rep       Date:  2017-01-06       Impact factor: 4.379

8.  Catechol glucosides act as donor/acceptor substrates of glucansucrase enzymes of Lactobacillus reuteri.

Authors:  Evelien M Te Poele; Vincent Valk; Tim Devlamynck; Sander S van Leeuwen; Lubbert Dijkhuizen
Journal:  Appl Microbiol Biotechnol       Date:  2017-03-03       Impact factor: 4.813

9.  Characterization of the Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer products representing a new glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes.

Authors:  Joana Gangoiti; Lisa Lamothe; Sander Sebastiaan van Leeuwen; Christina Vafiadi; Lubbert Dijkhuizen
Journal:  PLoS One       Date:  2017-04-11       Impact factor: 3.240

10.  Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB.

Authors:  Joana Gangoiti; Sander S van Leeuwen; Xiangfeng Meng; Stéphane Duboux; Christina Vafiadi; Tjaard Pijning; Lubbert Dijkhuizen
Journal:  Sci Rep       Date:  2017-08-30       Impact factor: 4.379
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.