Literature DB >> 18313038

Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme.

Sander S van Leeuwen1, Slavko Kralj, Ineke H van Geel-Schutten, Gerrit J Gerwig, Lubbert Dijkhuizen, Johannis P Kamerling.   

Abstract

The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1-->3,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows: [Formula: see text].

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Year:  2008        PMID： 18313038     DOI： 10.1016/j.carres.2008.01.042

Source DB:  PubMed          Journal:  Carbohydr Res        ISSN： 0008-6215            Impact factor:   2.104


  16 in total

1.  4,6-α-glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families 13 and 70.

Authors:  Slavko Kralj; Pieter Grijpstra; Sander S van Leeuwen; Hans Leemhuis; Justyna M Dobruchowska; Rachel M van der Kaaij; Amarila Malik; Ariyanti Oetari; Johannis P Kamerling; Lubbert Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  2011-09-23       Impact factor: 4.792

2.  Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes.

Authors:  Andreja Vujicic-Zagar; Tjaard Pijning; Slavko Kralj; Cesar A López; Wieger Eeuwema; Lubbert Dijkhuizen; Bauke W Dijkstra
Journal:  Proc Natl Acad Sci U S A       Date:  2010-11-30       Impact factor: 11.205

3.  Residue Leu940 has a crucial role in the linkage and reaction specificity of the glucansucrase GTF180 of the probiotic bacterium Lactobacillus reuteri 180.

Authors:  Xiangfeng Meng; Justyna M Dobruchowska; Tjaard Pijning; Cesar A López; Johannis P Kamerling; Lubbert Dijkhuizen
Journal:  J Biol Chem       Date:  2014-10-06       Impact factor: 5.157

4.  Characterization of the Functional Roles of Amino Acid Residues in Acceptor-binding Subsite +1 in the Active Site of the Glucansucrase GTF180 from Lactobacillus reuteri 180.

Authors:  Xiangfeng Meng; Tjaard Pijning; Justyna M Dobruchowska; Gerrit J Gerwig; Lubbert Dijkhuizen
Journal:  J Biol Chem       Date:  2015-10-27       Impact factor: 5.157

5.  Combinatorial engineering of dextransucrase specificity.

Authors:  Romain Irague; Laurence Tarquis; Isabelle André; Claire Moulis; Sandrine Morel; Pierre Monsan; Gabrielle Potocki-Véronèse; Magali Remaud-Siméon
Journal:  PLoS One       Date:  2013-10-18       Impact factor: 3.240

6.  Structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of Lactobacillus reuteri.

Authors:  Xiangfeng Meng; Tjaard Pijning; Justyna M Dobruchowska; Huifang Yin; Gerrit J Gerwig; Lubbert Dijkhuizen
Journal:  Sci Rep       Date:  2016-10-17       Impact factor: 4.379

Review 7.  Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.

Authors:  Xiangfeng Meng; Joana Gangoiti; Yuxiang Bai; Tjaard Pijning; Sander S Van Leeuwen; Lubbert Dijkhuizen
Journal:  Cell Mol Life Sci       Date:  2016-05-07       Impact factor: 9.261

8.  4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H.

Authors:  Joana Gangoiti; Sander S van Leeuwen; Gerrit J Gerwig; Stéphane Duboux; Christina Vafiadi; Tjaard Pijning; Lubbert Dijkhuizen
Journal:  Sci Rep       Date:  2017-01-06       Impact factor: 4.379

9.  Structure and biosynthesis of two exopolysaccharides produced by Lactobacillus johnsonii FI9785.

Authors:  Enes Dertli; Ian J Colquhoun; A Patrick Gunning; Roy J Bongaerts; Gwénaëlle Le Gall; Boyan B Bonev; Melinda J Mayer; Arjan Narbad
Journal:  J Biol Chem       Date:  2013-09-09       Impact factor: 5.157

10.  Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB.

Authors:  Joana Gangoiti; Sander S van Leeuwen; Xiangfeng Meng; Stéphane Duboux; Christina Vafiadi; Tjaard Pijning; Lubbert Dijkhuizen
Journal:  Sci Rep       Date:  2017-08-30       Impact factor: 4.379
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