Literature DB >> 1831011

The pyruvate kinase-coupled assay for ATPases: a critical analysis.

W T Jenkins1.   

Abstract

The pyruvate kinase assay for ATPases suffers from major systematic errors due to the variable amounts of the inhibitory product, ADP, present as the rates change. A simple method is presented to estimate the true uninhibited rates and the ADP inhibition constant for the ATPase.

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Year:  1991        PMID： 1831011     DOI： 10.1016/0003-2697(91)90160-u

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Comparison of bioluminescent kinase assays using substrate depletion and product formation.

Authors:  Cordelle Tanega; Min Shen; Bryan T Mott; Craig J Thomas; Ryan MacArthur; James Inglese; Douglas S Auld
Journal:  Assay Drug Dev Technol       Date:  2009-12       Impact factor: 1.738

2.  ATP-competitive, marine derived natural products that target the DEAD box helicase, eIF4A.

Authors:  Joseph Tillotson; Magdalena Kedzior; Larissa Guimarães; Alison B Ross; Tara L Peters; Andrew J Ambrose; Cody J Schmidlin; Donna D Zhang; Letícia V Costa-Lotufo; Abimael D Rodríguez; Jonathan H Schatz; Eli Chapman
Journal:  Bioorg Med Chem Lett       Date:  2017-07-19       Impact factor: 2.823

3.  Characterization of a vacuolar pyrophosphatase in Trypanosoma brucei and its localization to acidocalcisomes.

Authors:  C O Rodrigues; D A Scott; R Docampo
Journal:  Mol Cell Biol       Date:  1999-11       Impact factor: 4.272

4.  Unique helicase determinants in the essential conjugative TraI factor from Salmonella enterica serovar Typhimurium plasmid pCU1.

Authors:  Krystle J McLaughlin; Rebekah P Nash; Mathew R Redinbo
Journal:  J Bacteriol       Date:  2014-06-16       Impact factor: 3.490

5.  Preclinical anticancer activity of the potent, oral Src inhibitor AZD0530.

Authors:  Tim P Green; Mike Fennell; Robin Whittaker; Jon Curwen; Vivien Jacobs; Jack Allen; Armelle Logie; Judith Hargreaves; D Mark Hickinson; Robert W Wilkinson; Paul Elvin; Brigitte Boyer; Neil Carragher; Patrick A Plé; Alun Bermingham; Geoffrey A Holdgate; Walter H J Ward; Laurent F Hennequin; Barry R Davies; Gerard F Costello
Journal:  Mol Oncol       Date:  2009-02-07       Impact factor: 6.603

6.  Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase, and PI5P4Kα Lipid Kinase.

Authors:  Mindy I Davis; Douglas S Auld; James Inglese
Journal:  Methods Mol Biol       Date:  2016

7.  The anti-cancer IgM monoclonal antibody PAT-SM6 binds with high avidity to the unfolded protein response regulator GRP78.

Authors:  Zachary Rosenes; Terrence D Mulhern; Danny M Hatters; Leodevico L Ilag; Barbara E Power; Chris Hosking; Frank Hensel; Geoffrey J Howlett; Yee-Foong Mok
Journal:  PLoS One       Date:  2012-09-19       Impact factor: 3.240
  7 in total

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