Literature DB >> 18295723

Combination of poly(ethylenimine) and chitosan induces high gene transfection efficiency and low cytotoxicity.

Qing-Qing Zhao1, Jin-Liang Chen, Min Han, Wen-Quan Liang, Yasuhiko Tabata, Jian-Qing Gao.   

Abstract

In this study, the cytotoxicity and transfection efficiency of using chitosan/DNA complex combined with poly(ethylenimine) (PEI) were investigated. The combination of PEI with the chitosan/DNA complex markedly enhanced the gene expression of HeLa cells to 1000-fold of that induced by chitosan alone. PEI's cytotoxicity was considerably decreased upon combination with the chitosan/DNA complex. Furthermore, the PEI/chitosan/DNA could maintain the gene expression efficiency in the presence of serum.

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Year:  2008        PMID： 18295723     DOI： 10.1263/jbb.105.65

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  6 in total

1.  Chitosan/pshRNA plasmid nanoparticles targeting MDR1 gene reverse paclitaxel resistance in ovarian cancer cells.

Authors:  Yan Yang; Zehua Wang; Minfang Li; Shi Lu
Journal:  J Huazhong Univ Sci Technolog Med Sci       Date:  2009-04-28

2.  PEI-PEG-Chitosan Copolymer Coated Iron Oxide Nanoparticles for Safe Gene Delivery: synthesis, complexation, and transfection.

Authors:  Forrest M Kievit; Omid Veiseh; Narayan Bhattarai; Chen Fang; Jonathan W Gunn; Donghoon Lee; Richard G Ellenbogen; James M Olson; Miqin Zhang
Journal:  Adv Funct Mater       Date:  2009-07-24       Impact factor: 18.808

3.  Chitosan-graft-polyethylenimine/DNA nanoparticles as novel non-viral gene delivery vectors targeting osteoarthritis.

Authors:  Huading Lu; Yuhu Dai; Lulu Lv; Huiqing Zhao
Journal:  PLoS One       Date:  2014-01-02       Impact factor: 3.240

Review 4.  Intestinal Bacteria Encapsulated by Biomaterials Enhance Immunotherapy.

Authors:  Yilun Liu; Zhongmin Li; Yuanyu Wu; Xiabin Jing; Lin Li; Xuedong Fang
Journal:  Front Immunol       Date:  2021-02-10       Impact factor: 7.561

5.  The absence of CpG in plasmid DNA-chitosan polyplexes enhances transfection efficiencies and reduces inflammatory responses in murine lungs.

Authors:  Amaraporn Wongrakpanich; Andrea Adamcakova-Dodd; Wei Xie; Vijaya B Joshi; Kranti A Mapuskar; Sean M Geary; Douglas R Spitz; Peter S Thorne; Aliasger K Salem
Journal:  Mol Pharm       Date:  2014-02-04       Impact factor: 4.939

6.  Mannosylated chitosan nanoparticles for delivery of antisense oligonucleotides for macrophage targeting.

Authors:  Gyati Shilakari Asthana; Abhay Asthana; Dharm Veer Kohli; Suresh Prasad Vyas
Journal:  Biomed Res Int       Date:  2014-06-26       Impact factor: 3.411
  6 in total

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