Literature DB >> 18294072

Production and characterization of monoclonal antibody to botulinum neurotoxin type B light chain by phage display.

Myung-Shin Lee1, Ji Chul Lee, Cha Yong Choi, Junho Chung.   

Abstract

A monoclonal antibody to the light chain of botulinum neurotoxin type B (BoNT/B) was generated and its protective activity was evaluated in vivo. A chimeric rabbit/human Fab library was generated using bone marrow and spleen cDNAs of rabbits immunized with the BoNT/B light chain, and three monoclonal antibodies specific to the catalytic domain of BoNT/B were isolated. One of these clones, BCXRH1, was specific to a conformation-dependent epitope, and partially neutralized the BoNT/B complex in vivo.

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Year:  2008        PMID： 18294072     DOI： 10.1089/hyb.2007.0532

Source DB:  PubMed          Journal:  Hybridoma (Larchmt)        ISSN： 1554-0014


  6 in total

1.  High-throughput retrieval of physical DNA for NGS-identifiable clones in phage display library.

Authors:  Jinsung Noh; Okju Kim; Yushin Jung; Haejun Han; Jung-Eun Kim; Soohyun Kim; Sanghyub Lee; Jaeseong Park; Rae Hyuck Jung; Sang Il Kim; Jaejun Park; Jerome Han; Hyunho Lee; Duck Kyun Yoo; Amos C Lee; Euijin Kwon; Taehoon Ryu; Junho Chung; Sunghoon Kwon
Journal:  MAbs       Date:  2019-02-12       Impact factor: 5.857

2.  Gremlin-1 induces BMP-independent tumor cell proliferation, migration, and invasion.

Authors:  Minsoo Kim; Soomin Yoon; Sukmook Lee; Seon Ah Ha; Hyun Kee Kim; Jin Woo Kim; Junho Chung
Journal:  PLoS One       Date:  2012-04-13       Impact factor: 3.240

3.  An antibody reactive to the Gly63-Lys68 epitope of NT-proBNP exhibits O-glycosylation-independent binding.

Authors:  Yujean Lee; Hyori Kim; Junho Chung
Journal:  Exp Mol Med       Date:  2014-09-19       Impact factor: 8.718

Review 4.  From rabbit antibody repertoires to rabbit monoclonal antibodies.

Authors:  Justus Weber; Haiyong Peng; Christoph Rader
Journal:  Exp Mol Med       Date:  2017-03-24       Impact factor: 8.718

5.  Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents.

Authors:  Jerry O Conway; Laura J Sherwood; M Thelma Collazo; John A Garza; Andrew Hayhurst
Journal:  PLoS One       Date:  2010-01-21       Impact factor: 3.240

6.  A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System.

Authors:  Seohee Chang; Soohyun Kim; Jerome Han; Suji Ha; Hyunho Lee; Seo Woo Song; Daewon Lee; Sunghoon Kwon; Junho Chung; Junhoi Kim
Journal:  Biomolecules       Date:  2020-03-29
  6 in total

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