Literature DB >> 18294070

Construction of recombinant mouse IgG1 antibody directed against varicella zoster virus immediate early protein 63.

Niklaus H Mueller1, Laurie L Graf, Andrew J Shearer, Gregory P Owens, Donald H Gilden, Randall J Cohrs.   

Abstract

Five varicella zoster virus (VZV) genes are known to be transcribed in latently infected human ganglia. Transcripts from VZV gene 63, which encodes an immediate early (IE) protein, are the most prevalent and abundant. To obtain a reagent that might facilitate studies of the role of the IE63 protein in latency and reactivation, we selected an IE63-specific Fab fragment from a phage library and used it to prepare a recombinant mouse IgG1 antibody that detects IE63 and functions in Western blot, immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assays.

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Year:  2008        PMID： 18294070      PMCID： PMC2938744          DOI： 10.1089/hyb.2007.0530

Source DB:  PubMed          Journal:  Hybridoma (Larchmt)        ISSN： 1554-0014


  26 in total

1.  Varicella-Zoster virus gene expression in latently infected rat dorsal root ganglia.

Authors:  P G Kennedy; E Grinfeld; S Bontems; C Sadzot-Delvaux
Journal:  Virology       Date:  2001-10-25       Impact factor: 3.616

2.  Varicella-zoster virus ORF63 inhibits apoptosis of primary human neurons.

Authors:  Chantelle Hood; Anthony L Cunningham; Barry Slobedman; Ann M Arvin; Marvin H Sommer; Paul R Kinchington; Allison Abendroth
Journal:  J Virol       Date:  2006-01       Impact factor: 5.103

3.  Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3' to 5' exonuclease activity.

Authors:  Z Wang; M Raifu; M Howard; L Smith; D Hansen; R Goldsby; D Ratner
Journal:  J Immunol Methods       Date:  2000-01-13       Impact factor: 2.303

4.  Prevalence and abundance of latently transcribed varicella-zoster virus genes in human ganglia.

Authors:  Randall J Cohrs; Donald H Gilden
Journal:  J Virol       Date:  2006-12-27       Impact factor: 5.103

5.  Laser-capture microdissection of plasma cells from subacute sclerosing panencephalitis brain reveals intrathecal disease-relevant antibodies.

Authors:  Mark P Burgoon; Kathryne M Keays; Gregory P Owens; Alanna M Ritchie; Pradeep R Rai; Carlyne D Cool; Donald H Gilden
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-09       Impact factor: 11.205

6.  Cell-type-dependent activation of the cellular EF-1alpha promoter by the varicella-zoster virus IE63 protein.

Authors:  Tricia Zuranski; Hesham Nawar; Donna Czechowski; Jennifer M Lynch; Ann Arvin; John Hay; William T Ruyechan
Journal:  Virology       Date:  2005-07-20       Impact factor: 3.616

7.  Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex.

Authors:  Emmanuel Di Valentin; Sébastien Bontems; Lionel Habran; Olivier Jolois; Nicolas Markine-Goriaynoff; Alain Vanderplasschen; Catherine Sadzot-Delvaux; Jacques Piette
Journal:  Biol Chem       Date:  2005-03       Impact factor: 3.915

8.  Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus by intracellular detection of cytokine expression.

Authors:  H Asanuma; M Sharp; H T Maecker; V C Maino; A M Arvin
Journal:  J Infect Dis       Date:  2000-03       Impact factor: 5.226

Review 9.  The role of varicella zoster virus immediate-early proteins in latency and their potential use as components of vaccines.

Authors:  C Sadzot-Delvaux; B Rentier
Journal:  Arch Virol Suppl       Date:  2001

10.  The varicella-zoster virus-mediated delayed host shutoff: open reading frame 17 has no major function, whereas immediate-early 63 protein represses heterologous gene expression.

Authors:  Nathalie Desloges; Markus Rahaus; Manfred H Wolff
Journal:  Microbes Infect       Date:  2005-07-01       Impact factor: 2.700
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  3 in total

1.  Recombinant monoclonal antibody recognizes a unique epitope on varicella-zoster virus immediate-early 63 protein.

Authors:  Niklaus H Mueller; Nathan L Bos; Scott Seitz; Mary Wellish; Ravi Mahalingam; Don Gilden; Randall J Cohrs
Journal:  J Virol       Date:  2012-03-21       Impact factor: 5.103

2.  Application of antibody phage display to identify potential antigenic neural precursor cell proteins.

Authors:  Ioannis Paspaltsis; Evangelia Kesidou; Olga Touloumi; Roza Lagoudaki; Marina Boziki; Martina Samiotaki; Dimitra Dafou; Theodoros Sklaviadis; Nikolaos Grigoriadis
Journal:  J Biol Res (Thessalon)       Date:  2020-08-02       Impact factor: 1.889

3.  Phosphorylation of the nuclear form of varicella-zoster virus immediate-early protein 63 by casein kinase II at serine 186.

Authors:  Niklaus H Mueller; Laurie L Graf; David Orlicky; Don Gilden; Randall J Cohrs
Journal:  J Virol       Date:  2009-09-16       Impact factor: 5.103
  3 in total

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