AIMS: To examine the value of the DNase test as an alternative procedure for differentiating Corynebacterium diphtheriae from Corynebacterium-like colonies. METHODS AND RESULTS: DNase test medium was inoculated by spotting a loopful of bacterial growth and incubated aerobically at 37 degrees C. The DNase production was detectable following both 24 and 48 h incubation periods. The DNase activity was detected in all 91 C. diphtheriae (37 toxigenic and 54 nontoxigenic) strains examined, previously identified by both conventional biochemical methods and API Coryne System. Conversely, DNase test results were negative in 93.9% of the 564 nondiphtherial Gram-positive rod clinical strains. CONCLUSIONS: The DNase test emerged as an easily interpretable and cost-effective alternative screening procedure for C. diphtheriae laboratory identification. SIGNIFICANCE AND IMPACT OF THE STUDY: The method should facilitate routine laboratory diagnosis of toxigenic and nontoxigenic C. diphtheriae.
AIMS: To examine the value of the DNase test as an alternative procedure for differentiating Corynebacterium diphtheriae from Corynebacterium-like colonies. METHODS AND RESULTS: DNase test medium was inoculated by spotting a loopful of bacterial growth and incubated aerobically at 37 degrees C. The DNase production was detectable following both 24 and 48 h incubation periods. The DNase activity was detected in all 91 C. diphtheriae (37 toxigenic and 54 nontoxigenic) strains examined, previously identified by both conventional biochemical methods and API Coryne System. Conversely, DNase test results were negative in 93.9% of the 564 nondiphtherial Gram-positive rod clinical strains. CONCLUSIONS: The DNase test emerged as an easily interpretable and cost-effective alternative screening procedure for C. diphtheriae laboratory identification. SIGNIFICANCE AND IMPACT OF THE STUDY: The method should facilitate routine laboratory diagnosis of toxigenic and nontoxigenic C. diphtheriae.
Authors: Luciene de Fátima Costa Torres; Dayana Ribeiro; Raphael Hirata; Luis Gustavo Carvalho Pacheco; Monica Cristina Souza; Louisy Sanches dos Santos; Cíntia Silva dos Santos; Mohammad Salah; Mateus Matiuzzi da Costa; Marcio Garcia Ribeiro; Salah A Selim; Vasco Ariston de Carvalho Azevedo; Ana Luiza Mattos-Guaraldi Journal: Mem Inst Oswaldo Cruz Date: 2013-05 Impact factor: 2.743
Authors: L S Santos; L O Sant'anna; J N Ramos; E M Ladeira; R Stavracakis-Peixoto; L L G Borges; C S Santos; F Napoleão; T C F Camello; G A Pereira; R Hirata; V V Vieira; L M S S Cosme; P S Sabbadini; A L Mattos-Guaraldi Journal: Epidemiol Infect Date: 2015-03 Impact factor: 4.434
Authors: Robert C Shields; Norehan Mokhtar; Michael Ford; Michael J Hall; J Grant Burgess; Mohamed Reda ElBadawey; Nicholas S Jakubovics Journal: PLoS One Date: 2013-02-18 Impact factor: 3.240