BACKGROUND: We have investigated how recirculating lymphocytes patrol the liver in a normal steady state. METHODS: Thoracic duct lymphocytes of congeneic rats were intravenously transferred to host rats and donor cell trafficking in the liver and hepatic lymph was examined. Host hepatic lymph nodes (HLNs) were selectively removed, which allowed liver-derived donor cells to collect in the thoracic duct without transit in the intervening HLNs. RESULTS: The number of donor cells in the thoracic duct lymph significantly increased over the baseline 3, 5 and 11 h after transfer in the HLN-removed, non-pretreated, and HLN-ligated (in which a lymph efflux was blocked) groups, respectively. Histologically, donor cells appeared in the portal area from 0.5 h after transfer and frequently attached to the basal lamina of portal vein both externally and internally. Three hours after transfer, a few donor cells appeared in the subcapsular sinus of HLNs. CONCLUSION: The minimal transit time of rat recirculating lymphocytes is 3-4 h in the liver and 5-8 h in the hepatic LNs, in a normal steady state. Recirculating lymphocytes might transmigrate through the portal vein as well as the sinusoid in the periportal zone. This rapid transit might enable an efficient surveillance of the liver portal area by the recirculating lymphocytes.
BACKGROUND: We have investigated how recirculating lymphocytes patrol the liver in a normal steady state. METHODS: Thoracic duct lymphocytes of congeneic rats were intravenously transferred to host rats and donor cell trafficking in the liver and hepatic lymph was examined. Host hepatic lymph nodes (HLNs) were selectively removed, which allowed liver-derived donor cells to collect in the thoracic duct without transit in the intervening HLNs. RESULTS: The number of donor cells in the thoracic duct lymph significantly increased over the baseline 3, 5 and 11 h after transfer in the HLN-removed, non-pretreated, and HLN-ligated (in which a lymph efflux was blocked) groups, respectively. Histologically, donor cells appeared in the portal area from 0.5 h after transfer and frequently attached to the basal lamina of portal vein both externally and internally. Three hours after transfer, a few donor cells appeared in the subcapsular sinus of HLNs. CONCLUSION: The minimal transit time of rat recirculating lymphocytes is 3-4 h in the liver and 5-8 h in the hepatic LNs, in a normal steady state. Recirculating lymphocytes might transmigrate through the portal vein as well as the sinusoid in the periportal zone. This rapid transit might enable an efficient surveillance of the liver portal area by the recirculating lymphocytes.
Authors: Patricia F Lalor; John Herbert; Roy Bicknell; David H Adams Journal: Am J Physiol Gastrointest Liver Physiol Date: 2012-12-20 Impact factor: 4.052
Authors: Shishir Shetty; Christopher J Weston; Ye H Oo; Nina Westerlund; Zania Stamataki; Janine Youster; Stefan G Hubscher; Marko Salmi; Sirpa Jalkanen; Patricia F Lalor; David H Adams Journal: J Immunol Date: 2011-03-02 Impact factor: 5.422
Authors: Daniel A Patten; Garrick K Wilson; Dalan Bailey; Robert K Shaw; Sirpa Jalkanen; Marko Salmi; Antal Rot; Chris J Weston; David H Adams; Shishir Shetty Journal: Hepatology Date: 2016-11-25 Impact factor: 17.425
Authors: Sumera Karim; Evaggelia Liaskou; Samuel Hadley; Janine Youster; Jeff Faint; David H Adams; Patricia F Lalor Journal: Toxicol Sci Date: 2013-01-08 Impact factor: 4.849