| Literature DB >> 18288819 |
John C Sharpe1, John S Mitchell, Ling Lin, Nemanya Sedoglavich, Richard J Blaikie.
Abstract
A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody-gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18288819 DOI： 10.1021/ac702555r
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986