Literature DB >> 18287634

Methodologies for staining and visualisation of beta-galactosidase in mouse embryos and tissues.

Siobhan Loughna1, Deborah Henderson.   

Abstract

This chapter provides information on beta-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of beta-galactosidase can be visualized throughout development and in adult tissues. beta-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.

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Year:  2007        PMID: 18287634     DOI: 10.1007/978-1-59745-549-7_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  11 in total

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2.  Comments on Methods to Suppress Endogenous β-Galactosidase Activity in Mouse Tissues Expressing the LacZ Reporter Gene.

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Journal:  Proc Natl Acad Sci U S A       Date:  2021-03-02       Impact factor: 11.205

4.  Essential role of Wtip in mouse development and maintenance of the glomerular filtration barrier.

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5.  Nogo-B receptor deficiency causes cerebral vasculature defects during embryonic development in mice.

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6.  Left cardiac isomerism in the Sonic hedgehog null mouse.

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Authors:  Lucy Bradshaw; Bill Chaudhry; Victoria Hildreth; Sandra Webb; Deborah J Henderson
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9.  Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs.

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10.  The osteogenic cell surface marker BRIL/IFITM5 is dispensable for bone development and homeostasis in mice.

Authors:  Alexa Patoine; Abdallah Husseini; Bahar Kasaai; Marie-Hélène Gaumond; Pierre Moffatt
Journal:  PLoS One       Date:  2017-09-07       Impact factor: 3.240

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