AIMS/HYPOTHESIS: In patients with type 2 diabetes, reduced levels of circulating endothelial progenitor cells have been reported and these have been correlated with disease severity. In this study, we examined a panel of markers widely used to identify progenitor and/or stem cells, and determined their association with disease severity in diabetic patients. Since expression of chemokine (C-X-C motif) receptor 4 (CXCR4) has been associated with mobilisation and recruitment of progenitor cells, CXCR4 expression was also analysed. METHODS: Peripheral blood mononuclear cells (PBMCs) from 98 patients with type 2 diabetes and 39 control individuals were analysed by flow cytometry for surface marker expression. RESULTS: Cells expressing different combinations of progenitor and/or stem cell markers were severely reduced in PBMCs of diabetic patients compared with those of control participants. Moreover, a number of these putative progenitor cell populations were negatively associated with disease severity. Reduced expression of CXCR4 and CD34/CXCR4-positive cells was also observed in diabetic patients. PBMCs expressing CXCR4 positively correlated with levels of progenitor cells in control participants but not in diabetic patients. Levels of putative progenitor and CXCR4-positive cells were further decreased in patients with diabetic complications, including cardiovascular and microvascular diseases. CONCLUSIONS/ INTERPRETATION: A generalised decrease in a range of progenitor cell populations was observed in type 2 diabetic patients. This reduction was also negatively associated with disease severity.
AIMS/HYPOTHESIS: In patients with type 2 diabetes, reduced levels of circulating endothelial progenitor cells have been reported and these have been correlated with disease severity. In this study, we examined a panel of markers widely used to identify progenitor and/or stem cells, and determined their association with disease severity in diabeticpatients. Since expression of chemokine (C-X-C motif) receptor 4 (CXCR4) has been associated with mobilisation and recruitment of progenitor cells, CXCR4 expression was also analysed. METHODS: Peripheral blood mononuclear cells (PBMCs) from 98 patients with type 2 diabetes and 39 control individuals were analysed by flow cytometry for surface marker expression. RESULTS: Cells expressing different combinations of progenitor and/or stem cell markers were severely reduced in PBMCs of diabeticpatients compared with those of control participants. Moreover, a number of these putative progenitor cell populations were negatively associated with disease severity. Reduced expression of CXCR4 and CD34/CXCR4-positive cells was also observed in diabeticpatients. PBMCs expressing CXCR4 positively correlated with levels of progenitor cells in control participants but not in diabeticpatients. Levels of putative progenitor and CXCR4-positive cells were further decreased in patients with diabetic complications, including cardiovascular and microvascular diseases. CONCLUSIONS/ INTERPRETATION: A generalised decrease in a range of progenitor cell populations was observed in type 2 diabeticpatients. This reduction was also negatively associated with disease severity.
Authors: Per M Humpert; Renate Neuwirth; Marco J Battista; Olga Voronko; Maximilian von Eynatten; Ilze Konrade; Gottfried Rudofsky; Thoralf Wendt; Andreas Hamann; Michael Morcos; Peter P Nawroth; Angelika Bierhaus Journal: Diabetes Care Date: 2005-04 Impact factor: 19.112
Authors: Jason M Butler; Steven M Guthrie; Mehmet Koc; Aqeela Afzal; Sergio Caballero; H Logan Brooks; Robert N Mames; Mark S Segal; Maria B Grant; Edward W Scott Journal: J Clin Invest Date: 2005-01 Impact factor: 14.808
Authors: Katherine A Gallagher; Zhao-Jun Liu; Min Xiao; Haiying Chen; Lee J Goldstein; Donald G Buerk; April Nedeau; Stephen R Thom; Omaida C Velazquez Journal: J Clin Invest Date: 2007-05 Impact factor: 14.808
Authors: Gian Paolo Fadini; Elisa Boscaro; Saula de Kreutzenberg; Carlo Agostini; Florian Seeger; Stefanie Dimmeler; Andreas Zeiher; Antonio Tiengo; Angelo Avogaro Journal: Diabetes Care Date: 2010-02-11 Impact factor: 19.112
Authors: Ashay D Bhatwadekar; E P Guerin; Yagna P R Jarajapu; Sergio Caballero; Carl Sheridan; David Kent; Laurence Kennedy; M Cecilia Lansang; Frank W Ruscetti; Carl J Pepine; Paul J Higgins; Stephen H Bartelmez; Maria B Grant Journal: Diabetes Date: 2010-05-11 Impact factor: 9.461