R Bluma1, M R Amaiden, J Daghero, M Etcheverry. 1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales. Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
Abstract
AIMS: The antifungal effect of Pimpinella anisum (anise), Pëumus boldus (boldus), Mentha piperita (peppermint), Origanum vulgare (oregano) and Minthosthachys verticillata (peperina) essential oils against Aspergillus section Flavi (two isolates of Aspergillus parasiticus and two isolates of Aspergillus flavus) was evaluated in maize meal extract agar at 0.982 and 0.955 water activities, at 25 degrees C. METHODS AND RESULTS: The percentage of germination, germ-tube elongation rate, growth rate and aflatoxin B(1) (AFB(1)) accumulation at different essential oils concentrations were evaluated. Anise and boldus essential oils were the most inhibitory at 500 mg kg(-1) to all growth parameters of the fungus. These essential oils inhibited the percentage of germination, germ-tube elongation rate and fungal growth. AFB(1) accumulation was completely inhibited by anise, boldus and oregano essential oils. Peperina and peppermint essential oils inhibited AFB(1) production by 85-90% in all concentrations assayed. CONCLUSIONS: Anise and boldus essential oils could be considered as effective fungitoxicans for Aspergillus section flavi. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results suggest that these phytochemical compounds could be used alone or in conjunction with other substances to control the presence of aflatoxigenic fungi in stored maize.
AIMS: The antifungal effect of Pimpinella anisum (anise), Pëumus boldus (boldus), Mentha piperita (peppermint), Origanum vulgare (oregano) and Minthosthachys verticillata (peperina) essential oils against Aspergillus section Flavi (two isolates of Aspergillus parasiticus and two isolates of Aspergillus flavus) was evaluated in maize meal extract agar at 0.982 and 0.955 water activities, at 25 degrees C. METHODS AND RESULTS: The percentage of germination, germ-tube elongation rate, growth rate and aflatoxin B(1) (AFB(1)) accumulation at different essential oils concentrations were evaluated. Anise and boldus essential oils were the most inhibitory at 500 mg kg(-1) to all growth parameters of the fungus. These essential oils inhibited the percentage of germination, germ-tube elongation rate and fungal growth. AFB(1) accumulation was completely inhibited by anise, boldus and oreganoessential oils. Peperina and peppermintessential oils inhibited AFB(1) production by 85-90% in all concentrations assayed. CONCLUSIONS:Anise and boldus essential oils could be considered as effective fungitoxicans for Aspergillus section flavi. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results suggest that these phytochemical compounds could be used alone or in conjunction with other substances to control the presence of aflatoxigenic fungi in stored maize.
Authors: Khaled Hosny; Hani Asfour; Waleed Rizg; Nabil A Alhakamy; Amal Sindi; Hala Alkhalidi; Walaa Abualsunun; Rana Bakhaidar; Alshaimaa M Almehmady; Sara Akeel; Sarah Ali; Adel Alghaith; Sultan Alshehri; Rasha Khallaf Journal: Int J Nanomedicine Date: 2021-08-13
Authors: Miroslava Hlebová; Lukas Hleba; Juraj Medo; Viktoria Uzsakova; Pavel Kloucek; Matej Bozik; Peter Haščík; Juraj Čuboň Journal: Foods Date: 2021-12-04