| Literature DB >> 18279908 |
Ross Stevenson1, Helen C Baxter, Alastair Aitken, Tom Brown, Robert L Baxter.
Abstract
A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.Entities:
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Year: 2008 PMID： 18279908 DOI： 10.1016/j.bioorg.2007.12.005
Source DB: PubMed Journal: Bioorg Chem ISSN： 0045-2068 Impact factor: 5.275