Lie Xia1, Long Jiang, Ya-qin Zhu. 1. Department of Operative Dentistry & Endodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Abstract
PURPOSE: To compare the cytotoxicity of three endodontic materials (MTA, Dycal and GIC) on human periodontal ligament cells (HPDLCs) in vitro. METHODS: HPDLCs proliferation was evaluated using methylthiazol tetrazolium (MTT) assay for three endodontic materials in different concentration. SAS6.12 software package was used to analyse the results. RESULTS: There was no statistically significant difference in three subgroups of MTA group (P>0.05). Statistically significant differences were found in Dycal group (P<0.05). In GIC group, there was no statistically significant difference between the control and 10 mg/ml subgroup (P>0.05). At the same time, statistically significant differences were found in between the control and 20 mg/ml or 40 mg/ml subgroup (P<0.05). CONCLUSION: Dycal and GIC are more cytotoxic than MTA. MTA is superior to Dycal and GIC in biocompatibility.
PURPOSE: To compare the cytotoxicity of three endodontic materials (MTA, Dycal and GIC) on human periodontal ligament cells (HPDLCs) in vitro. METHODS: HPDLCs proliferation was evaluated using methylthiazol tetrazolium (MTT) assay for three endodontic materials in different concentration. SAS6.12 software package was used to analyse the results. RESULTS: There was no statistically significant difference in three subgroups of MTA group (P>0.05). Statistically significant differences were found in Dycal group (P<0.05). In GIC group, there was no statistically significant difference between the control and 10 mg/ml subgroup (P>0.05). At the same time, statistically significant differences were found in between the control and 20 mg/ml or 40 mg/ml subgroup (P<0.05). CONCLUSION: Dycal and GIC are more cytotoxic than MTA. MTA is superior to Dycal and GIC in biocompatibility.