Literature DB >> 18275187

Hidden electronic excited state of enhanced green fluorescent protein.

Haruko Hosoi1, Shoichi Yamaguchi, Hideaki Mizuno, Atsushi Miyawaki, Tahei Tahara.   

Abstract

Precise two-photon absorption spectra of the green fluorescent protein (GFP) and the mutants sapphire-GFP (T203I) and enhanced GFP (S65T/F64L), as well as a model compound for the chromophore, 4'-hydroxybenzylidene-2,3-dimethylimidazolinone (HBDI) were measured by multiplex two-photon absorption spectroscopy. The observed TPA bands of the anionic forms of enhanced GFP and HBDI were significantly shifted to the higher energy compared with the lowest-energy bands in one-photon absorption spectra. This result indicated the existence of a hidden electronic excited state in the vicinity of the lowest excited singlet (S1) state of the anionic form of the GFP chromophore, which is the origin of the blue shift of the two-photon absorption spectra as well as two-photon fluorescence excitation spectra.

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Year:  2008        PMID： 18275187     DOI： 10.1021/jp711628u

Source DB:  PubMed          Journal:  J Phys Chem B        ISSN： 1520-5207            Impact factor:   2.991


  10 in total

1.  Subdiffraction-limit two-photon fluorescence microscopy for GFP-tagged cell imaging.

Authors:  Qifeng Li; Sherry S H Wu; Keng C Chou
Journal:  Biophys J       Date:  2009-12-16       Impact factor: 4.033

Review 2.  Quantitative two-photon imaging of fluorescent biosensors.

Authors:  Gary Yellen; Rebecca Mongeon
Journal:  Curr Opin Chem Biol       Date:  2015-06-12       Impact factor: 8.822

3.  Describing two-photon absorptivity of fluorescent proteins with a new vibronic coupling mechanism.

Authors:  M Drobizhev; N S Makarov; S E Tillo; T E Hughes; A Rebane
Journal:  J Phys Chem B       Date:  2012-01-30       Impact factor: 2.991

Review 4.  Two-photon absorption properties of fluorescent proteins.

Authors:  Mikhail Drobizhev; Nikolay S Makarov; Shane E Tillo; Thomas E Hughes; Aleksander Rebane
Journal:  Nat Methods       Date:  2011-04-28       Impact factor: 28.547

5.  A new approach to dual-color two-photon microscopy with fluorescent proteins.

Authors:  Shane E Tillo; Thomas E Hughes; Nikolay S Makarov; Aleks Rebane; Mikhail Drobizhev
Journal:  BMC Biotechnol       Date:  2010-02-02       Impact factor: 2.563

6.  Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins.

Authors:  M Drobizhev; S Tillo; N S Makarov; T E Hughes; A Rebane
Journal:  J Phys Chem B       Date:  2009-01-29       Impact factor: 2.991

7.  Two-photon conversion of a bacterial phytochrome.

Authors:  Serge G Sokolovski; Evgeny A Zherebtsov; Rajiv K Kar; David Golonka; Robert Stabel; Nikolai B Chichkov; Andrei Gorodetsky; Igor Schapiro; Andreas Möglich; Edik U Rafailov
Journal:  Biophys J       Date:  2021-02-03       Impact factor: 4.033

8.  Isoenergetic two-photon excitation enhances solvent-to-solute excited-state proton transfer.

Authors:  Jurick Lahiri; Mehdi Moemeni; Jessica Kline; Ilias Magoulas; Stephen H Yuwono; Maryann Laboe; Jun Shen; Babak Borhan; Piotr Piecuch; James E Jackson; G J Blanchard; Marcos Dantus
Journal:  J Chem Phys       Date:  2020-12-14       Impact factor: 3.488

9.  Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy.

Authors:  Keisuke Isobe; Hiroshi Hashimoto; Akira Suda; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa
Journal:  Biomed Opt Express       Date:  2010-08-23       Impact factor: 3.732

10.  What is the Optimal Size of the Quantum Region in Embedding Calculations of Two-Photon Absorption Spectra of Fluorescent Proteins?

Authors:  Dawid Grabarek; Tadeusz Andruniów
Journal:  J Chem Theory Comput       Date:  2020-09-21       Impact factor: 6.006
  10 in total

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