Jun Lin1, You-qin Li, Wan-hong Li, Hong Chen. 1. Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. junlin2@126.com
Abstract
OBJECTIVE: To observe the effects of calcium phosphate cement/eucommia ulmoides extracts compound (CPC/EUE) on cell proliferation and adhesion of MC3T3E1 osteoblasts in vitro. METHODS: Cultured MC3T3E1 osteoblasts were divided into 5 groups: blank control, CPC/EUE 0, 0.152, 0.304 and 0.608 mg/ml. 3H-TdR and scan electronic microscopy (SEM) were used to observe the cell growth, proliferation and adhesion. RESULT: The CPC/EUE compound enhanced the cell growth, proliferation and adhesion. The concentrations of 0.152 mg/ml, 0.304 mg/ml were more significant in enhancing the cell proliferation rate (P<0.05). CONCLUSION: CPC/EUE can promote the cell growth, proliferation and adhesion of the osteoblasts.
OBJECTIVE: To observe the effects of calcium phosphate cement/eucommia ulmoides extracts compound (CPC/EUE) on cell proliferation and adhesion of MC3T3E1 osteoblasts in vitro. METHODS: Cultured MC3T3E1 osteoblasts were divided into 5 groups: blank control, CPC/EUE 0, 0.152, 0.304 and 0.608 mg/ml. 3H-TdR and scan electronic microscopy (SEM) were used to observe the cell growth, proliferation and adhesion. RESULT: The CPC/EUE compound enhanced the cell growth, proliferation and adhesion. The concentrations of 0.152 mg/ml, 0.304 mg/ml were more significant in enhancing the cell proliferation rate (P<0.05). CONCLUSION: CPC/EUE can promote the cell growth, proliferation and adhesion of the osteoblasts.