Literature DB >> 18273816

Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites.

I G Hamoy1, E J M Santos, S E B Santos.   

Abstract

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18273816     DOI： 10.4238/vol7-1gmr394

Source DB:  PubMed          Journal:  Genet Mol Res        ISSN： 1676-5680


  5 in total

1.  High genetic diversity and connectivity in Colossoma macropomum in the Amazon basin revealed by microsatellite markers.

Authors:  Paola Fazzi-Gomes; Sávio Guerreiro; Glauber David Almeida Palheta; Nuno Filipe Alves Correa de Melo; Sidney Santos; Igor Hamoy
Journal:  Genet Mol Biol       Date:  2017-02-06       Impact factor: 1.771

2.  Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins.

Authors:  Lucas Simon Torati; John Bernard Taggart; Eduardo Sousa Varela; Juliana Araripe; Stefanie Wehner; Hervé Migaud
Journal:  BMC Genet       Date:  2019-01-28       Impact factor: 2.797

3.  Low Genetic Diversity and Structuring of the Arapaima (Osteoglossiformes, Arapaimidae) Population of the Araguaia-Tocantins Basin.

Authors:  Carla A Vitorino; Fabrícia Nogueira; Issakar L Souza; Juliana Araripe; Paulo C Venere
Journal:  Front Genet       Date:  2017-10-24       Impact factor: 4.599

4.  Genomic approach for conservation and the sustainable management of endangered species of the Amazon.

Authors:  Paola Fazzi-Gomes; Jonas Aguiar; Gleyce Fonseca Cabral; Diego Marques; Helber Palheta; Fabiano Moreira; Marilia Rodrigues; Renata Cavalcante; Jorge Souza; Caio Silva; Igor Hamoy; Sidney Santos
Journal:  PLoS One       Date:  2021-02-24       Impact factor: 3.240

5.  Novel Microsatellite Markers Used for Determining Genetic Diversity and Tracing of Wild and Farmed Populations of the Amazonian Giant Fish Arapaima gigas.

Authors:  Paola Fabiana Fazzi-Gomes; Jonas da Paz Aguiar; Diego Marques; Gleyce Fonseca Cabral; Fabiano Cordeiro Moreira; Marilia Danyelle Nunes Rodrigues; Caio Santos Silva; Igor Hamoy; Sidney Santos
Journal:  Genes (Basel)       Date:  2021-08-27       Impact factor: 4.096
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.