Chuan-Xiang Xu1, Hong-Tao Liu, Jing Wang. 1. Institute of Healthy and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Abstract
OBJECTIVE: To investigate changes of 5-hydroxytryptamine (5-HT) and its synthesis rate-limiting enzyme tryptophan hydroxylase (TPH) in the ventral horn of spinal cord after exercise-induced fatigue, and to further discuss the mechanism of exercise-induced central fatigue at spinal level. METHODS: Sixteen healthy adult Wistar rats were randomly divided into 2 groups: exercise-induced fatigue group and control group. Immunohistochemical staining for 5-HT and TPH in the ventral horn were performed and analyzed quantitatively. The mean optic densities of 5-HT and TPH positive fibers or terminals were measured by computerized image analyzer. RESULTS: Both 5-HT and TPH positive fibers/terminals decreased in the exercise-induced fatigue group. The immunohistochemical staining was weaker and the mean optic densities decreased obviously in the fatigue group compared with those in the control group (P< 0.05). CONCLUSION: 5-HT and TPH in the ventral horn of spinal cord might be involved in exercise-induced fatigue.
OBJECTIVE: To investigate changes of 5-hydroxytryptamine (5-HT) and its synthesis rate-limiting enzyme tryptophan hydroxylase (TPH) in the ventral horn of spinal cord after exercise-induced fatigue, and to further discuss the mechanism of exercise-induced central fatigue at spinal level. METHODS: Sixteen healthy adult Wistar rats were randomly divided into 2 groups: exercise-induced fatigue group and control group. Immunohistochemical staining for 5-HT and TPH in the ventral horn were performed and analyzed quantitatively. The mean optic densities of 5-HT and TPH positive fibers or terminals were measured by computerized image analyzer. RESULTS: Both 5-HT and TPH positive fibers/terminals decreased in the exercise-induced fatigue group. The immunohistochemical staining was weaker and the mean optic densities decreased obviously in the fatigue group compared with those in the control group (P< 0.05). CONCLUSION: 5-HT and TPH in the ventral horn of spinal cord might be involved in exercise-induced fatigue.
Authors: Benjamin Heng; Gilles J Guillemin; Bahar Kavyani; Brett A Lidbury; Richard Schloeffel; Paul R Fisher; Daniel Missailidis; Sarah J Annesley; Mona Dehhaghi Journal: Cell Mol Life Sci Date: 2022-07-11 Impact factor: 9.207