OBJECTIVE: The purpose was to investigate the expression of musashi-1 (msi-1) and its significances in small intestinal mucosa that was severely damaged by high-dose 5-FU. METHODS: A total of 40 adult C57BL/6J mice were divided into two groups: the control group (n = 8, group A) and experimental group (n = 32). The mice in the control group were treated with PBS by intraperitoneal injection, and the other mice were treated with high-dose 5-FU (150 mg/kg body weight for 5 consecutive days) by intraperitoneal injection. At the 1st (group B), 3rd (group C) and 5th (group D) day after treatment with high-dose 5-FU, the dying mice were killed, HE staining and immunohistochemical techniques were used to detect the expression of the putative marker of intestinal epithelial stem cells, msi-1, in samples of the middle intestine from these mice, and the percentage of the msi-1-positive cells from the intestinal mucosal cells of the mice in group B was detected by FACS. RESULTS: After treatment with high-dose 5-FU, the intestinal mucosa suffered severe damage: the villi and crypts disappeared, the number of msi-1-positive cells increased greatly, the intestinal epithelial cells could be divided into two fractions by FACS, and the percentage of msi-1-positive cells was up to 67.75% in the fraction in which the value of FSC was higher. CONCLUSIONS: After treatment with high-dose 5-FU, the percentage of intestinal stem cells had increased significantly, which was useful for the further isolation and enrichment of intestinal epithelial stem cells.
OBJECTIVE: The purpose was to investigate the expression of musashi-1 (msi-1) and its significances in small intestinal mucosa that was severely damaged by high-dose 5-FU. METHODS: A total of 40 adult C57BL/6J mice were divided into two groups: the control group (n = 8, group A) and experimental group (n = 32). The mice in the control group were treated with PBS by intraperitoneal injection, and the other mice were treated with high-dose 5-FU (150 mg/kg body weight for 5 consecutive days) by intraperitoneal injection. At the 1st (group B), 3rd (group C) and 5th (group D) day after treatment with high-dose 5-FU, the dying mice were killed, HE staining and immunohistochemical techniques were used to detect the expression of the putative marker of intestinal epithelial stem cells, msi-1, in samples of the middle intestine from these mice, and the percentage of the msi-1-positive cells from the intestinal mucosal cells of the mice in group B was detected by FACS. RESULTS: After treatment with high-dose 5-FU, the intestinal mucosa suffered severe damage: the villi and crypts disappeared, the number of msi-1-positive cells increased greatly, the intestinal epithelial cells could be divided into two fractions by FACS, and the percentage of msi-1-positive cells was up to 67.75% in the fraction in which the value of FSC was higher. CONCLUSIONS: After treatment with high-dose 5-FU, the percentage of intestinal stem cells had increased significantly, which was useful for the further isolation and enrichment of intestinal epithelial stem cells.
Authors: K Liadaki; A T Kho; D Sanoudou; J Schienda; A Flint; A H Beggs; I S Kohane; L M Kunkel Journal: Exp Cell Res Date: 2004-11-11 Impact factor: 3.905
Authors: Christopher S Potten; Catherine Booth; Gregory L Tudor; Dawn Booth; Gerard Brady; Patricia Hurley; Gary Ashton; Robert Clarke; Shin-ichi Sakakibara; Hideyuki Okano Journal: Differentiation Date: 2003-01 Impact factor: 3.880
Authors: Dat T Vo; Dharmalingam Subramaniam; Marc Remke; Tarea L Burton; Philip J Uren; Jonathan A Gelfond; Raquel de Sousa Abreu; Suzanne C Burns; Mei Qiao; Uthra Suresh; Andrey Korshunov; Adrian M Dubuc; Paul A Northcott; Andrew D Smith; Stefan M Pfister; Michael D Taylor; Sarath C Janga; Shrikant Anant; Christine Vogel; Luiz O F Penalva Journal: Am J Pathol Date: 2012-09-14 Impact factor: 4.307
Authors: F M Cambuli; B R Correa; A Rezza; S C Burns; M Qiao; P J Uren; E Kress; A Boussouar; P A F Galante; L O F Penalva; M Plateroti Journal: Stem Cells Date: 2015-09-26 Impact factor: 6.277
Authors: Jennyfer M García-Cárdenas; Santiago Guerrero; Andrés López-Cortés; Isaac Armendáriz-Castillo; Patricia Guevara-Ramírez; Andy Pérez-Villa; Verónica Yumiceba; Ana Karina Zambrano; Paola E Leone; César Paz-Y-Miño Journal: Front Mol Biosci Date: 2019-08-07