Literature DB >> 18270658

The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems.

Marcie H Moehnke1, Terumi Midoro-Horiuti, Randall M Goldblum, Christopher M Kearney.   

Abstract

Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 microg/g leaf material) and Pichia pastoris (100 microg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.

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Year:  2008        PMID： 18270658      PMCID： PMC2588349          DOI： 10.1007/s10529-008-9665-x

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  13 in total

1.  Variable expression of pathogenesis-related protein allergen in mountain cedar (Juniperus ashei) pollen.

Authors:  T Midoro-Horiuti; R M Goldblum; A Kurosky; T G Wood; E G Brooks
Journal:  J Immunol       Date:  2000-02-15       Impact factor: 5.422

Review 2.  Regulation of specific immune responses by chemical and structural modifications of allergens.

Authors:  C A Akdis; K Blaser
Journal:  Int Arch Allergy Immunol       Date:  2000-04       Impact factor: 2.749

3.  Rapid production of recombinant allergens in Nicotiana benthamiana and their impact on diagnosis and therapy.

Authors:  H Breiteneder; M Krebitz; U Wiedermann; B Wagner; D Essl; H Steinkellner; T H Turpen; C Ebner; D Buck; B Niggemann; O Scheiner
Journal:  Int Arch Allergy Immunol       Date:  2001 Jan-Mar       Impact factor: 2.749

4.  Rapid production of the major birch pollen allergen Bet v 1 in Nicotiana benthamiana plants and its immunological in vitro and in vivo characterization.

Authors:  M Krebitz; U Wiedermann; D Essl; H Steinkellner; B Wagner; T H Turpen; C Ebner; O Scheiner; H Breiteneder
Journal:  FASEB J       Date:  2000-07       Impact factor: 5.191

5.  Mountain cedar allergens found in nonpollen tree parts.

Authors:  D W Goetz; M A Goetz; B A Whisman
Journal:  Ann Allergy Asthma Immunol       Date:  1995-09       Impact factor: 6.347

6.  Homology modeling and characterization of IgE binding epitopes of mountain cedar allergen Jun a 3.

Authors:  K V Soman; T Midoro-Horiuti; J C Ferreon; R M Goldblum; E G Brooks; A Kurosky; W Braun; C H Schein
Journal:  Biophys J       Date:  2000-09       Impact factor: 4.033

Review 7.  Recombinant expression systems for allergen vaccines.

Authors:  Mohan B Singh; Prem L Bhalla
Journal:  Inflamm Allergy Drug Targets       Date:  2006-01

8.  Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: detection of partial oxidation of methionine by NMR.

Authors:  Patricia Barral; María Luisa Tejera; Miguel Angel Treviño; Eva Batanero; Mayte Villalba; Marta Bruix; Rosalía Rodríguez
Journal:  Protein Expr Purif       Date:  2004-10       Impact factor: 1.650

9.  Assessment of recombinant dog allergens Can f 1 and Can f 2 for the diagnosis of dog allergy.

Authors:  S Saarelainen; A Taivainen; M Rytkönen-Nissinen; S Auriola; A Immonen; R Mäntyjärvi; J Rautiainen; T Kinnunen; T Virtanen
Journal:  Clin Exp Allergy       Date:  2004-10       Impact factor: 5.018

Review 10.  The future of antigen-specific immunotherapy of allergy.

Authors:  Rudolf Valenta
Journal:  Nat Rev Immunol       Date:  2002-06       Impact factor: 53.106
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  4 in total

1.  History of the biomedical studies PhD program: a joint graduate program of the Baylor Health Care system and Baylor University.

Authors:  Christine R Morel; Joshua M Horton; Han Peng; Kangling Xu; Sushil K Batra; Jonathan P Miles; Robert R Kane
Journal:  Proc (Bayl Univ Med Cent)       Date:  2008-10

2.  Dunaliella as an attractive candidate for molecular farming.

Authors:  Abolfazl Barzegari; Mohammad Amin Hejazi; Nahid Hosseinzadeh; Solat Eslami; Elnaz Mehdizadeh Aghdam; Mohammad Saeid Hejazi
Journal:  Mol Biol Rep       Date:  2009-11-27       Impact factor: 2.316

3.  Plant-expressed recombinant mountain cedar allergen Jun a 1 is allergenic and has limited pectate lyase activity.

Authors:  Zun Liu; Shikha Bhattacharyya; Bo Ning; Terumi Midoro-Horiuti; Edmund W Czerwinski; Randall M Goldblum; Andrew Mort; Christopher M Kearney
Journal:  Int Arch Allergy Immunol       Date:  2010-06-17       Impact factor: 2.749

4.  Heterologous Expression of PKPI and Pin1 Proteinase Inhibitors Enhances Plant Fitness and Broad-Spectrum Resistance to Biotic Threats.

Authors:  David Turrà; Stefania Vitale; Roberta Marra; Sheridan L Woo; Matteo Lorito
Journal:  Front Plant Sci       Date:  2020-04-30       Impact factor: 5.753
  4 in total

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