| Literature DB >> 18270028 |
Abstract
Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.Mesh：
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Year: 2008 PMID： 18270028 DOI： 10.1109/TBME.2007.914403
Source DB: PubMed Journal: IEEE Trans Biomed Eng ISSN： 0018-9294 Impact factor: 4.538