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Literature DB >> 1826827

Expression of the NS1 gene of dengue virus type 2 using vaccinia virus. Dimerisation of the NS1 glycoprotein.

Abstract

A part of the genome of dengue virus type 2 spanning the coding region from the carboxy terminus of the envelope protein E to the beginning of the NS3 protein was expressed using recombinant vaccinia virus. Additional constructs which contained open reading frames terminating within the NS1 or NS2A genes were also expressed. NS1 dimers were formed by extended NS1 molecules containing 61 amino acids of NS2A. No dimers were detected when NS1 was shortened by 79 amino acids at its carboxy terminus.

Entities: Gene Species

Mesh：

Substances：

Year: 1991 PMID： 1826827 DOI： 10.1007/bf01310771

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

27 in total

1. Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization.

Authors: G Winkler; S E Maxwell; C Ruemmler; V Stollar
Journal: Virology Date: 1989-07 Impact factor: 3.616

2. Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2a.

Authors: B Falgout; R Chanock; C J Lai
Journal: J Virol Date: 1989-05 Impact factor: 5.103

Review 3. Viral proteins containing the purine NTP-binding sequence pattern.

Authors: A E Gorbalenya; E V Koonin
Journal: Nucleic Acids Res Date: 1989-11-11 Impact factor: 16.971

4. Nucleotide sequence of dengue 2 RNA and comparison of the encoded proteins with those of other flaviviruses.

Authors: Y S Hahn; R Galler; T Hunkapiller; J M Dalrymple; J H Strauss; E G Strauss
Journal: Virology Date: 1988-01 Impact factor: 3.616

5. Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer.

Authors: G Winkler; V B Randolph; G R Cleaves; T E Ryan; V Stollar
Journal: Virology Date: 1988-01 Impact factor: 3.616

6. Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural protein NS1.

Authors: J J Schlesinger; M W Brandriss; C B Cropp; T P Monath
Journal: J Virol Date: 1986-12 Impact factor: 5.103

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

8. Synthesis of proteins and glycoproteins in dengue type 2 virus-infected vero and Aedes albopictus cells.

Authors: G W Smith; P J Wright
Journal: J Gen Virol Date: 1985-03 Impact factor: 3.891

9. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.

Authors: M Mackett; G L Smith; B Moss
Journal: J Virol Date: 1984-03 Impact factor: 5.103

10. Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions.

Authors: C M Rice; C A Franke; J H Strauss; D E Hruby
Journal: J Virol Date: 1985-10 Impact factor: 5.103

7 in total

1. Characterization of a siberian virus isolated from a patient with progressive chronic tick-borne encephalitis.

Authors: T S Gritsun; T V Frolova; A I Zhankov; M Armesto; S L Turner; M P Frolova; V V Pogodina; V A Lashkevich; E A Gould
Journal: J Virol Date: 2003-01 Impact factor: 5.103

2. Comparative analysis of the NS 1 gene sequences of dengue-1 viruses prototype Hawaii strain and Thai isolate TH-Sman, and determination of the intratypic variation of NS 1 protein among dengue-1 viruses.

Authors: S Y Shiu; K W Ip; E A Gould; K M Chan
Journal: Arch Virol Date: 1993 Impact factor: 2.574