G S Anisha1, R K Sukumaran, P Prema. 1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (Formerly Regional Research Laboratory), CSIR, Trivandrum, Kerala, India.
Abstract
AIM: The present study was aimed at evaluating the effects of the three crucial factors, galactose concentration, inoculum size and moisture content, on alpha-galactosidase production by the filamentous actinobacterium Streptomyces griseoloalbus in solid-state fermentation. METHODS AND RESULTS: Central Composite design was adopted to derive a statistical model for the optimization of fermentation conditions. Maximum alpha-galactosidase yield (117 U g(-1) of dry fermented substrate) was obtained when soya bean flour supplemented with 1.5% galactose and with initial moisture content of 40% was inoculated with 1.9 x 10(6) CFU g(-1) initial dry substrate. CONCLUSIONS: The model was valid and could result in considerably enhanced enzyme yield. SIGNIFICANCE AND IMPACT OF THE STUDY: The results indicated a cost effective method for the production of alpha-galactosidase using soya bean flour. This is the first report on exploitation of the potential of filamentous bacterium for the production of alpha-galactosidase, an enzyme having versatile applications.
AIM: The present study was aimed at evaluating the effects of the three crucial factors, galactose concentration, inoculum size and moisture content, on alpha-galactosidase production by the filamentous actinobacteriumStreptomyces griseoloalbus in solid-state fermentation. METHODS AND RESULTS: Central Composite design was adopted to derive a statistical model for the optimization of fermentation conditions. Maximum alpha-galactosidase yield (117 U g(-1) of dry fermented substrate) was obtained when soya bean flour supplemented with 1.5% galactose and with initial moisture content of 40% was inoculated with 1.9 x 10(6) CFU g(-1) initial dry substrate. CONCLUSIONS: The model was valid and could result in considerably enhanced enzyme yield. SIGNIFICANCE AND IMPACT OF THE STUDY: The results indicated a cost effective method for the production of alpha-galactosidase using soya bean flour. This is the first report on exploitation of the potential of filamentous bacterium for the production of alpha-galactosidase, an enzyme having versatile applications.
Authors: M Siddique Awan; Nabila Tabbasam; N Ayub; M E Babar; Shahid Mahboob Rana; M I Rajoka Journal: Mol Biol Rep Date: 2010-07-15 Impact factor: 2.316