AIMS: Several cases of campylobacteriosis reported worldwide seemingly conflict with the strict growth requirements and sensitivity to environmental stress of Campylobacter jejuni. In this study, the need for a micro-aerobic environment [dissolved oxygen tension (DOT): 0.1-90%; 100% air saturation)] and the adaptive responses to oxygen stress were studied. METHODS AND RESULTS: The growth of C. jejuni in continuous culture was assessed under different DOT in the presence or absence of pyruvate. In a medium without pyruvate, continuous cultures of C. jejuni showed typically micro-aerobic behaviour and cells were unable to grow under fully aerobic conditions. However in the presence of pyruvate (25 mmol l(-1)), continuous cultures of C. jejuni were able to grow in a broad DOT range, varying from 0.1% to at least 90%, and the catalase activity was decreased. CONCLUSIONS: Addition of pyruvate results in the decrease in the concentration of hydrogen peroxide, which enables C. jejuni to grow aerobically. SIGNIFICANCE AND IMPACT OF THE STUDY: New information on the oxidative physiology of C. jejuni and its ability to grow aerobically in media supplemented with pyruvate is presented.
AIMS: Several cases of campylobacteriosis reported worldwide seemingly conflict with the strict growth requirements and sensitivity to environmental stress of Campylobacter jejuni. In this study, the need for a micro-aerobic environment [dissolved oxygen tension (DOT): 0.1-90%; 100% air saturation)] and the adaptive responses to oxygen stress were studied. METHODS AND RESULTS: The growth of C. jejuni in continuous culture was assessed under different DOT in the presence or absence of pyruvate. In a medium without pyruvate, continuous cultures of C. jejuni showed typically micro-aerobic behaviour and cells were unable to grow under fully aerobic conditions. However in the presence of pyruvate (25 mmol l(-1)), continuous cultures of C. jejuni were able to grow in a broad DOT range, varying from 0.1% to at least 90%, and the catalase activity was decreased. CONCLUSIONS: Addition of pyruvate results in the decrease in the concentration of hydrogen peroxide, which enables C. jejuni to grow aerobically. SIGNIFICANCE AND IMPACT OF THE STUDY: New information on the oxidative physiology of C. jejuni and its ability to grow aerobically in media supplemented with pyruvate is presented.
Authors: Kristoffer T Baek; Christina S Vegge; Joanna Skórko-Glonek; Lone Brøndsted Journal: Appl Environ Microbiol Date: 2010-11-12 Impact factor: 4.792
Authors: Canh Phung; Timothy B Wilson; José A Quinteros; Peter C Scott; Robert J Moore; Thi Thu Hao Van Journal: Sci Rep Date: 2021-10-21 Impact factor: 4.379