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Literature DB >> 182661

The demonstration of acid phosphatase in cultured 3T3 mouse cells.

Abstract

Acid phosphatase has been demonstrated ultrastructurally in 3T3 and SV40-3T3 mouse cells using sodium beta-glycerophosphate and p-nitrophenyl phosphate as substrate. The former substrate only demonstrates the enzyme in lysosomes and elements of the Golgi apparatus while the latter demonstrates it in the cisternae of the endoplasmic reticulum and in the cell surface as well as at lysosomal sites. The significance of surface acid phosphatase activity is discussed in terms of sublethal autolysis.

Entities: Chemical Species

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Year: 1976 PMID： 182661 DOI： 10.1007/bf00494554

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

18 in total

1. An evaluation of lysosomal enzyme leakage as a factor influencing the behaviour of transformed cells.

Authors: J D Elligsen; J E Thompson; H E Frey
Journal: Exp Cell Res Date: 1975-04 Impact factor: 3.905

2. A histochemical study of acid phosphatase in normal and virus-transformed cultured fibroblasts.

Authors: A L Dawson; D J Beadle; D C Livingston; S W Fisher
Journal: Histochem J Date: 1975-01

3. Reversible and irreversible effects of tissue culture on enzyme patterns of spontaneous mouse tumors and mouse and human embryo tissues.

Authors: P D Wilson
Journal: Cancer Res Date: 1973-02 Impact factor: 12.701

2. An ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth Galleria mellonella in vitro.

Authors: A F Rowley; N A Ratcliffe
Journal: Cell Tissue Res Date: 1979-06-08 Impact factor: 5.249

3. Altered distribution of acid phosphatase in neoplastic prostatic cells.

Authors: D R Fischer; W Gevers
Journal: Urol Res Date: 1980

3 in total

The demonstration of acid phosphatase in cultured 3T3 mouse cells.

1. An evaluation of lysosomal enzyme leakage as a factor influencing the behaviour of transformed cells.

2. A histochemical study of acid phosphatase in normal and virus-transformed cultured fibroblasts.

3. Reversible and irreversible effects of tissue culture on enzyme patterns of spontaneous mouse tumors and mouse and human embryo tissues.

4. Elevated glycosidases and proteolytic enzymes in cells transformed by RNA tumor virus.

5. Microsomal and lysosomal acid phosphatase isoenzymes of mouse kidney. Characterization and separation.

6. Changes in lysosomal associated structures in human fibroblasts kept in resting phase.

7. Histochemistry of non-specific phosphatases: the use of p-nitrophenyl phosphate and -glycerophosphate as substrates.

8. Glycoprotein degradation. Glycosidases in fibroblasts transformed by oncogenic viruses.

9. Rat liver acid phosphatase: differences in lysosomal and cytoplasmic forms.

10. Histochemical study of a number of hydrolases, including a new acid phosphatase, in various tissues of men and mice.

1. An ultrastructural study of phosphatases and aryl sulphatase in BHK and CHO cells grown in culture.

2. An ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth Galleria mellonella in vitro.

3. Altered distribution of acid phosphatase in neoplastic prostatic cells.