Literature DB >> 18266

Effect of metabolites on epsilon-N-hydroxylysine formation in cell-free extracts of Aerobacter aerogenes 62-1.

G J Murray, G E Clark, M A Parniak, T Viswanatha.   

Abstract

The conversion of L-lysine to its corresponding epsilon-N-hydroxy derivative has been achieved for the first time by cell-free extracts of Aerobacter aerogenes 62-1. Partial fractionation by differential centrifugation (at 12 000 X g) revealed that both supernatant and pellet are essential for maximum enzymatic activity. The omega-N-hydroxylase (EC 1.14.99) was found to function optimally at pH 7-7.5 and exhibited an apparent Km of about 75 muM for L-lysine. L(+)-Lactate or DL-lactate and pyruvate greatly stimulate the omega-N-hydroxylase activity. The system is strongly inhibited by arsenite and sulfite.

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Year:  1977        PMID： 18266     DOI： 10.1139/o77-090

Source DB:  PubMed          Journal:  Can J Biochem        ISSN： 0008-4018


  6 in total

1.  Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222.

Authors:  H J Plattner; P Pfefferle; A Romaguera; S Waschütza; H Diekmann
Journal:  Biol Met       Date:  1989

2.  Construction and biochemical characterization of recombinant cytoplasmic forms of the IucD protein (lysine:N6-hydroxylase) encoded by the pColV-K30 aerobactin gene cluster.

Authors:  A Thariath; D Socha; M A Valvano; T Viswanatha
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

3.  Genetic and biochemical characterization of the aerobactin synthesis operon on pColV.

Authors:  R Gross; F Engelbrecht; V Braun
Journal:  Mol Gen Genet       Date:  1984

4.  Cloning and nucleotide sequence of the pvdA gene encoding the pyoverdin biosynthetic enzyme L-ornithine N5-oxygenase in Pseudomonas aeruginosa.

Authors:  P Visca; A Ciervo; N Orsi
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

5.  Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.

Authors:  V de Lorenzo; A Bindereif; B H Paw; J B Neilands
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

6.  Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the synthesis of pyoverdin.

Authors:  P Visca; L Serino; N Orsi
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490
  6 in total

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