Literature DB >> 18265339

Whole-mount in situ hybridization and detection of RNAs in vertebrate embryos and isolated organs.

Anne Pizard1, Anna Haramis, Andrés E Carrasco, Paula Franco, Silvia López, Alejandra Paganelli.   

Abstract

Nonisotopic in situ hybridization using intact embryos or organs is an important method for determining the spatial distribution of RNAs. Because it allows the analysis of large numbers of samples, it is amenable to temporal expression studies and comparison between different genotypes. It offers sensitivity and reproducibility. In addition, histological details are not lost during the staining process. The protocols in this unit can be used for whole-mount in situ hybridization in Xenopus, mouse, and chicken embryos, as well as dissected organs from mouse and chicken. Preparation of digoxigenin-labeled riboprobes is also described.

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Year:  2004        PMID： 18265339     DOI： 10.1002/0471142727.mb1409s66

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  14 in total

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2.  FGF8 is essential for formation of the ductal system in the male reproductive tract.

Authors:  Jirouta Kitagaki; Yutaka Ueda; Xuan Chi; Nirmala Sharma; Cynthia M Elder; Erika Truffer; Frank Costantini; Mark Lewandoski; Alan O Perantoni
Journal:  Development       Date:  2011-12       Impact factor: 6.868

3.  Wnt4 induces nephronic tubules in metanephric mesenchyme by a non-canonical mechanism.

Authors:  Shunsuke Tanigawa; Honghe Wang; Yili Yang; Nirmala Sharma; Nadya Tarasova; Rieko Ajima; Terry P Yamaguchi; Luis G Rodriguez; Alan O Perantoni
Journal:  Dev Biol       Date:  2011-01-21       Impact factor: 3.582

4.  New candidate genes identified for controlling mouse gonadal sex determination and the early stages of granulosa and Sertoli cell differentiation.

Authors:  Gerrit J Bouma; Quanah J Hudson; Linda L Washburn; Eva M Eicher
Journal:  Biol Reprod       Date:  2009-10-28       Impact factor: 4.285

5.  Transcription of subtelomere tandemly repetitive DNA in chicken embryogenesis.

Authors:  Irina Trofimova; Darya Chervyakova; Alla Krasikova
Journal:  Chromosome Res       Date:  2015-09       Impact factor: 5.239

6.  Using whole mount in situ hybridization to link molecular and organismal biology.

Authors:  Nicole L Jacobs; R Craig Albertson; Jason R Wiles
Journal:  J Vis Exp       Date:  2011-03-31       Impact factor: 1.355

7.  FoxA4 favours notochord formation by inhibiting contiguous mesodermal fates and restricts anterior neural development in Xenopus embryos.

Authors:  Sabrina Murgan; Aitana Manuela Castro Colabianchi; Renato José Monti; Laura Elena Boyadjián López; Cecilia E Aguirre; Ernesto González Stivala; Andrés E Carrasco; Silvia L López
Journal:  PLoS One       Date:  2014-10-24       Impact factor: 3.240

8.  Abnormal placental development and early embryonic lethality in EpCAM-null mice.

Authors:  Keisuke Nagao; Jianjian Zhu; Mallorie B Heneghan; Jeffrey C Hanson; Maria I Morasso; Lino Tessarollo; Susan Mackem; Mark C Udey
Journal:  PLoS One       Date:  2009-12-31       Impact factor: 3.240

9.  An intact brachyury function is necessary to prevent spurious axial development in Xenopus laevis.

Authors:  Cecilia E Aguirre; Sabrina Murgan; Andrés E Carrasco; Silvia L López
Journal:  PLoS One       Date:  2013-01-24       Impact factor: 3.240

10.  Dissection and downstream analysis of zebra finch embryos at early stages of development.

Authors:  Jessica R Murray; Monika E Stanciauskas; Tejas S Aralere; Margaret S Saha
Journal:  J Vis Exp       Date:  2014-06-21       Impact factor: 1.355
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