Literature DB >> 18265204

Mouse embryonic stem (ES) cell culture.

D A Conner1.   

Abstract

Mouse embryonic stem (ES) cells are used to generate mouse mutants by gene targeting and blastocyst-mediated transgenesis. ES cells must be cultured under conditions that prevent differentiation to maintain their ability to transmit altered alleles by contributing to the germ line. This unit describes one of the most common methods for culturing ES cells using mouse embryo fibroblast (MEF) feeder layers and recombinant leukemia inhibitory factor (LIF) to prevent differentiation.

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Year:  2001        PMID： 18265204     DOI： 10.1002/0471142727.mb2303s51

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  6 in total

1.  G protein coupled receptors in embryonic stem cells: a role for Gs-alpha signaling.

Authors:  Brian T Layden; Marsha Newman; Fei Chen; Amanda Fisher; William L Lowe
Journal:  PLoS One       Date:  2010-02-08       Impact factor: 3.240

2.  Nrf2 Induces IL-17D to Mediate Tumor and Virus Surveillance.

Authors:  Robert Saddawi-Konefka; Ruth Seelige; Emilie T E Gross; Eric Levy; Stephen C Searles; Allen Washington; Endi K Santosa; Beichen Liu; Timothy E O'Sullivan; Olivier Harismendy; Jack D Bui
Journal:  Cell Rep       Date:  2016-08-18       Impact factor: 9.423

3.  Enhanced leptin sensitivity, reduced adiposity, and improved glucose homeostasis in mice lacking exchange protein directly activated by cyclic AMP isoform 1.

Authors:  Jingbo Yan; Fang C Mei; Hongqiang Cheng; Dieu Hung Lao; Yaohua Hu; Jingna Wei; Igor Patrikeev; Dapeng Hao; Sonja J Stutz; Kelly T Dineley; Massoud Motamedi; Jonathan D Hommel; Kathryn A Cunningham; Ju Chen; Xiaodong Cheng
Journal:  Mol Cell Biol       Date:  2012-12-21       Impact factor: 4.272

4.  Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA.

Authors:  Romualdas Vaisvila; V K Chaithanya Ponnaluri; Zhiyi Sun; Bradley W Langhorst; Lana Saleh; Shengxi Guan; Nan Dai; Matthew A Campbell; Brittany S Sexton; Katherine Marks; Mala Samaranayake; James C Samuelson; Heidi E Church; Esta Tamanaha; Ivan R Corrêa; Sriharsa Pradhan; Eileen T Dimalanta; Thomas C Evans; Louise Williams; Theodore B Davis
Journal:  Genome Res       Date:  2021-06-17       Impact factor: 9.043

5.  Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells.

Authors:  Mitsutoshi Yamada; Tohru Sugawara; Shinju Usami; Rina Nakanishi; Hidenori Akustu
Journal:  STAR Protoc       Date:  2021-09-11

Review 6.  Stem cells: An insight into the therapeutic aspects from medical and dental perspectives.

Authors:  Muniapillai Sivakumar; Janardhanam Dineshshankar; P M Sunil; R Madhavan Nirmal; J Sathiyajeeva; Balasubramanian Saravanan; A R Senthileagappan
Journal:  J Pharm Bioallied Sci       Date:  2015-08
  6 in total

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