Literature DB >> 18265203

Mouse embryo fibroblast (MEF) feeder cell preparation.

D A Conner1.   

Abstract

Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 18265203     DOI： 10.1002/0471142727.mb2302s51

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  49 in total

1.  Combining competition assays with genetic complementation strategies to dissect mouse embryonic stem cell self-renewal and pluripotency.

Authors:  Dung-Fang Lee; Jie Su; Ana Sevilla; Julian Gingold; Christoph Schaniel; Ihor R Lemischka
Journal:  Nat Protoc       Date:  2012-03-22       Impact factor: 13.491

2.  Hippo signaling is required for Notch-dependent smooth muscle differentiation of neural crest.

Authors:  Lauren J Manderfield; Haig Aghajanian; Kurt A Engleka; Lillian Y Lim; Feiyan Liu; Rajan Jain; Li Li; Eric N Olson; Jonathan A Epstein
Journal:  Development       Date:  2015-08-07       Impact factor: 6.868

3.  Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells.

Authors:  Jonghwan Kim; Alan B Cantor; Stuart H Orkin; Jianlong Wang
Journal:  Nat Protoc       Date:  2009-03-26       Impact factor: 13.491

4.  Generation of induced pluripotent stem cells from neural stem cells.

Authors:  Jeong Beom Kim; Holm Zaehres; Marcos J Araúzo-Bravo; Hans R Schöler
Journal:  Nat Protoc       Date:  2009-09-17       Impact factor: 13.491

5.  Generation of CD44 gene-deficient mouse derived induced pluripotent stem cells: CD44 gene-deficient iPSCs.

Authors:  Zhenwei Song; Qianqian Ji; Haijing Zhao; Yu Nie; Zuyong He; Yaosheng Chen; Peiqing Cong
Journal:  In Vitro Cell Dev Biol Anim       Date:  2014-06-21       Impact factor: 2.416

6.  Spatiotemporal expression of Math6 during mouse embryonic development.

Authors:  Baigang Wang; Ajeesh Balakrishnan-Renuka; Markus Napirei; Carsten Theiss; Beate Brand-Saberi
Journal:  Histochem Cell Biol       Date:  2015-01-13       Impact factor: 4.304

7.  CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells.

Authors:  Benjamin L Kidder
Journal:  J Vis Exp       Date:  2018-05-25       Impact factor: 1.355

8.  Contribution of hepatic lineage stage-specific donor memory to the differential potential of induced mouse pluripotent stem cells.

Authors:  Seung Bum Lee; Daekwan Seo; Dongho Choi; Kye-Yoon Park; Agnes Holczbauer; Jens U Marquardt; Elizabeth A Conner; Valentina M Factor; Snorri S Thorgeirsson
Journal:  Stem Cells       Date:  2012-05       Impact factor: 6.277

9.  Atg7 is required for acrosome biogenesis during spermatogenesis in mice.

Authors:  Hongna Wang; Haifeng Wan; Xixia Li; Weixiao Liu; Qi Chen; Yaqing Wang; Lin Yang; Hongmei Tang; Xiujun Zhang; Enkui Duan; Xiaoyang Zhao; Fei Gao; Wei Li
Journal:  Cell Res       Date:  2014-05-23       Impact factor: 25.617

10.  G protein coupled receptors in embryonic stem cells: a role for Gs-alpha signaling.

Authors:  Brian T Layden; Marsha Newman; Fei Chen; Amanda Fisher; William L Lowe
Journal:  PLoS One       Date:  2010-02-08       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.