Literature DB >> 18265179

Synthesis and purification of oligonucleotides.

A Ellington1, J D Pollard.   

Abstract

This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.

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Year:  2001        PMID： 18265179     DOI： 10.1002/0471142727.mb0211s42

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  2 in total

1.  Highly parallel oligonucleotide purification and functionalization using reversible chemistry.

Authors:  Kerri T York; Ryan C Smith; Rob Yang; Peter C Melnyk; Melissa M Wiley; Casey M Turk; Mostafa Ronaghi; Kevin L Gunderson; Frank J Steemers
Journal:  Nucleic Acids Res       Date:  2011-10-29       Impact factor: 16.971

2.  Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media.

Authors:  Shizuma Tanaka; Shinsuke Yukami; Yuhei Hachiro; Yuichi Ohya; Akinori Kuzuya
Journal:  Polymers (Basel)       Date:  2019-10-02       Impact factor: 4.329
  2 in total

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