Literature DB >> 18265160

Special considerations for glycolipids and their purification.

D F Smith1, P A Prieto.   

Abstract

This unit describes the antigenic stimulation of in vitro antibody production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro antibody production is presented along with a procedure for quantifying the number of antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described.

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Year:  2001        PMID： 18265160     DOI： 10.1002/0471142727.mb1703s22

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  7 in total

1.  Lactosomes: structural and compositional classification of unique nanometer-sized protein lipid particles of human milk.

Authors:  Nurit Argov-Argaman; Jennifer T Smilowitz; Daniel A Bricarello; Mariana Barboza; Larry Lerno; John W Froehlich; Hyeyoung Lee; Angela M Zivkovic; Danielle G Lemay; Samara Freeman; Carlito B Lebrilla; Atul N Parikh; J Bruce German
Journal:  J Agric Food Chem       Date:  2010-10-06       Impact factor: 5.279

2.  Rapid Profiling of Bovine and Human Milk Gangliosides by Matrix-Assisted Laser Desorption/Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.

Authors:  Hyeyoung Lee; Hyun Joo An; Larry A Lerno; J Bruce German; Carlito B Lebrilla
Journal:  Int J Mass Spectrom       Date:  2011-08-15       Impact factor: 1.986

3.  Development and characterization of a specific IgG monoclonal antibody toward the Lewis x antigen using splenocytes of Schistosoma mansoni-infected mice.

Authors:  Msano Mandalasi; Nelum Dorabawila; David F Smith; Jamie Heimburg-Molinaro; Richard D Cummings; A Kwame Nyame
Journal:  Glycobiology       Date:  2013-03-29       Impact factor: 4.313

4.  Mouse Siglec-1 Mediates trans-Infection of Surface-bound Murine Leukemia Virus in a Sialic Acid N-Acyl Side Chain-dependent Manner.

Authors:  Elina Erikson; Paul R Wratil; Martin Frank; Ina Ambiel; Katharina Pahnke; Maria Pino; Parastoo Azadi; Nuria Izquierdo-Useros; Javier Martinez-Picado; Chris Meier; Ronald L Schnaar; Paul R Crocker; Werner Reutter; Oliver T Keppler
Journal:  J Biol Chem       Date:  2015-09-14       Impact factor: 5.157

5.  Only α-Gal bound to lipids, but not to proteins, is transported across enterocytes as an IgE-reactive molecule that can induce effector cell activation.

Authors:  Patricia Román-Carrasco; Barbara Lieder; Veronika Somoza; Marta Ponce; Zsolt Szépfalusi; Diana Martin; Wolfgang Hemmer; Ines Swoboda
Journal:  Allergy       Date:  2019-07-16       Impact factor: 13.146

6.  High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma.

Authors:  Huan-Hsien Kuo; Ruey-Jen Lin; Jung-Tung Hung; Chung-Bao Hsieh; Tsai-Hsien Hung; Fei-Yun Lo; Ming-Yi Ho; Chau-Ting Yeh; Yen-Lin Huang; John Yu; Alice L Yu
Journal:  Sci Rep       Date:  2017-09-07       Impact factor: 4.379

Review 7.  Anomeric O-Functionalization of Carbohydrates for Chemical Conjugation to Vaccine Constructs.

Authors:  Simon S Park; Hsiao-Wu Hsieh; Jacquelyn Gervay-Hague
Journal:  Molecules       Date:  2018-07-17       Impact factor: 4.411
  7 in total

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