Literature DB >> 18265124

Generation of recombinant vaccinia viruses.

P L Earl1, B Moss, L S Wyatt, M W Carroll.   

Abstract

This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.

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Year:  2001        PMID： 18265124     DOI： 10.1002/0471142727.mb1617s43

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  46 in total

1.  Skin mast cells protect mice against vaccinia virus by triggering mast cell receptor S1PR2 and releasing antimicrobial peptides.

Authors:  Zhenping Wang; Yuping Lai; Jamie J Bernard; Daniel T Macleod; Anna L Cogen; Bernard Moss; Anna Di Nardo
Journal:  J Immunol       Date:  2011-12-02       Impact factor: 5.422

2.  Optimizing high dimensional gene expression studies for immune response following smallpox vaccination using Taqman® low density immune arrays.

Authors:  Ann L Oberg; Neelam Dhiman; Diane E Grill; Jenna E Ryan; Richard B Kennedy; Gregory A Poland
Journal:  J Immunol Methods       Date:  2011-01-28       Impact factor: 2.303

3.  Correlations between vaccinia-specific immune responses within a cohort of armed forces members.

Authors:  Benjamin J Umlauf; Inna G Ovsyannikova; Iana H Haralambieva; Richard B Kennedy; Robert A Vierkant; V Shane Pankratz; Robert M Jacobson; Gregory A Poland
Journal:  Viral Immunol       Date:  2011-09-29       Impact factor: 2.257

4.  Development of the small-molecule antiviral ST-246 as a smallpox therapeutic.

Authors:  Douglas W Grosenbach; Robert Jordan; Dennis E Hruby
Journal:  Future Virol       Date:  2011-05       Impact factor: 1.831

5.  Direct formation of vaccinia virus membranes from the endoplasmic reticulum in the absence of the newly characterized L2-interacting protein A30.5.

Authors:  Liliana Maruri-Avidal; Andrea S Weisberg; Bernard Moss
Journal:  J Virol       Date:  2013-09-11       Impact factor: 5.103

6.  Identification of 10 cowpox virus proteins that are necessary for induction of hemorrhagic lesions (red pocks) on chorioallantoic membranes.

Authors:  Zhiyong Xu; Dimitrios Zikos; Aistė Tamošiūnaitė; Robert Klopfleisch; Nikolaus Osterrieder; B Karsten Tischer
Journal:  J Virol       Date:  2014-05-21       Impact factor: 5.103

7.  Vaccinia virus virion membrane biogenesis protein A11 associates with viral membranes in a manner that requires the expression of another membrane biogenesis protein, A6.

Authors:  Xiang Wu; Xiangzhi Meng; Bo Yan; Lloyd Rose; Junpeng Deng; Yan Xiang
Journal:  J Virol       Date:  2012-08-08       Impact factor: 5.103

8.  Vaccinia Virus A6 Is a Two-Domain Protein Requiring a Cognate N-Terminal Domain for Full Viral Membrane Assembly Activity.

Authors:  Xiangzhi Meng; Lloyd Rose; Yue Han; Junpeng Deng; Yan Xiang
Journal:  J Virol       Date:  2017-04-28       Impact factor: 5.103

9.  Characterization of ectromelia virus deficient in EVM036, the homolog of vaccinia virus F13L, and its application for rapid generation of recombinant viruses.

Authors:  Felicia Roscoe; Ren-Huan Xu; Luis J Sigal
Journal:  J Virol       Date:  2012-10-03       Impact factor: 5.103

10.  Direct presentation is sufficient for an efficient anti-viral CD8+ T cell response.

Authors:  Ren-Huan Xu; Sanda Remakus; Xueying Ma; Felicia Roscoe; Luis J Sigal
Journal:  PLoS Pathog       Date:  2010-02-12       Impact factor: 6.823
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