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Literature DB >> 18265123

Preparation of cell cultures and vaccinia virus stocks.

P L Earl¹, N Cooper, L S Wyatt, B Moss, M W Carroll.

Abstract

This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for the titration of standard and MVA vaccinia virus stocks.

Entities: Disease Species

Mesh：

Year: 2001 PMID： 18265123 DOI： 10.1002/0471142727.mb1616s43

Source DB: PubMed Journal: Curr Protoc Mol Biol ISSN： 1934-3647

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Cited

34 in total

1. Skin mast cells protect mice against vaccinia virus by triggering mast cell receptor S1PR2 and releasing antimicrobial peptides.

Authors: Zhenping Wang; Yuping Lai; Jamie J Bernard; Daniel T Macleod; Anna L Cogen; Bernard Moss; Anna Di Nardo
Journal: J Immunol Date: 2011-12-02 Impact factor: 5.422

2. Optimizing high dimensional gene expression studies for immune response following smallpox vaccination using Taqman® low density immune arrays.

Authors: Ann L Oberg; Neelam Dhiman; Diane E Grill; Jenna E Ryan; Richard B Kennedy; Gregory A Poland
Journal: J Immunol Methods Date: 2011-01-28 Impact factor: 2.303

3. Correlations between vaccinia-specific immune responses within a cohort of armed forces members.

Authors: Benjamin J Umlauf; Inna G Ovsyannikova; Iana H Haralambieva; Richard B Kennedy; Robert A Vierkant; V Shane Pankratz; Robert M Jacobson; Gregory A Poland
Journal: Viral Immunol Date: 2011-09-29 Impact factor: 2.257

4. Development of the small-molecule antiviral ST-246 as a smallpox therapeutic.

Authors: Douglas W Grosenbach; Robert Jordan; Dennis E Hruby
Journal: Future Virol Date: 2011-05 Impact factor: 1.831

5. Direct formation of vaccinia virus membranes from the endoplasmic reticulum in the absence of the newly characterized L2-interacting protein A30.5.

Authors: Liliana Maruri-Avidal; Andrea S Weisberg; Bernard Moss
Journal: J Virol Date: 2013-09-11 Impact factor: 5.103

Preparation of cell cultures and vaccinia virus stocks.

1. Skin mast cells protect mice against vaccinia virus by triggering mast cell receptor S1PR2 and releasing antimicrobial peptides.

2. Optimizing high dimensional gene expression studies for immune response following smallpox vaccination using Taqman® low density immune arrays.

3. Correlations between vaccinia-specific immune responses within a cohort of armed forces members.

4. Development of the small-molecule antiviral ST-246 as a smallpox therapeutic.

5. Direct formation of vaccinia virus membranes from the endoplasmic reticulum in the absence of the newly characterized L2-interacting protein A30.5.

6. Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling.

7. Shortened Intervals during Heterologous Boosting Preserve Memory CD8 T Cell Function but Compromise Longevity.

8. Pervasive initiation and 3'-end formation of poxvirus postreplicative RNAs.

9. Duplication of the A17L locus of vaccinia virus provides an alternate route to rifampin resistance.

10. Direct presentation is sufficient for an efficient anti-viral CD8+ T cell response.