| Literature DB >> 18265109 |
Abstract
This unit describes fixation and staining for b-galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants. To help achieve consistent results with larger samples, an alternate protocol details preparation of thick sections. Support protocols are provided for clearing of tissues for better resolution and paraffin-embedding the whole embryo.Entities:
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Year: 2001 PMID： 18265109 DOI： 10.1002/0471142727.mb1414s50
Source DB: PubMed Journal: Curr Protoc Mol Biol ISSN： 1934-3647