Literature DB >> 18265080

Cloning of hybridoma cell lines by limiting dilution.

S A Fuller1, M Takahashi, J G Hurrell.   

Abstract

Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.

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Substances：

Year:  2001        PMID： 18265080     DOI： 10.1002/0471142727.mb1108s01

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  11 in total

1.  AAV vectors containing rDNA homology display increased chromosomal integration and transgene persistence.

Authors:  Zhongya Wang; Leszek Lisowski; Milton J Finegold; Hiroyuki Nakai; Mark A Kay; Markus Grompe
Journal:  Mol Ther       Date:  2012-09-18       Impact factor: 11.454

2.  Proteome alterations during clonal isolation of established human pancreatic cancer cell lines.

Authors:  P Bernhard; T Feilen; M Rogg; K Fröhlich; M Cosenza-Contreras; F Hause; C Schell; O Schilling
Journal:  Cell Mol Life Sci       Date:  2022-10-22       Impact factor: 9.207

3.  TLX1 (HOX11) immortalization of embryonic stem cell-derived and primary murine hematopoietic progenitors.

Authors:  Robert G Hawley; Teresa S Hawley; Alan B Cantor
Journal:  Curr Protoc Stem Cell Biol       Date:  2008-12

Review 4.  Single-cell RNA sequencing in Drosophila: Technologies and applications.

Authors:  Hongjie Li
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2020-09-16       Impact factor: 5.814

5.  An acidic microenvironment increases NK cell killing of Cryptococcus neoformans and Cryptococcus gattii by enhancing perforin degranulation.

Authors:  Anowara Islam; Shu Shun Li; Paul Oykhman; Martina Timm-McCann; Shaunna M Huston; Danuta Stack; Richard F Xiang; Margaret M Kelly; Christopher H Mody
Journal:  PLoS Pathog       Date:  2013-07-11       Impact factor: 6.823

6.  Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells.

Authors:  Jiexiong Xie; Isaura Christiaens; Bo Yang; Ivan Trus; Bert Devriendt; Tingting Cui; Ruifang Wei; Hans J Nauwynck
Journal:  Vet Res       Date:  2018-07-18       Impact factor: 3.683

Review 7.  Technologies for Single-Cell Isolation.

Authors:  Andre Gross; Jonas Schoendube; Stefan Zimmermann; Maximilian Steeb; Roland Zengerle; Peter Koltay
Journal:  Int J Mol Sci       Date:  2015-07-24       Impact factor: 5.923

8.  Ultra-sensitive detection of tumorigenic cellular impurities in human cell-processed therapeutic products by digital analysis of soft agar colony formation.

Authors:  Shinji Kusakawa; Satoshi Yasuda; Takuya Kuroda; Shin Kawamata; Yoji Sato
Journal:  Sci Rep       Date:  2015-12-08       Impact factor: 4.379

9.  Knocking out Ornithine Decarboxylase Antizyme 1 (OAZ1) Improves Recombinant Protein Expression in the HEK293 Cell Line.

Authors:  Laura Abaandou; Joseph Shiloach
Journal:  Med Sci (Basel)       Date:  2018-06-08

10.  Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression.

Authors:  Jan Zitzmann; Christine Schreiber; Joel Eichmann; Roberto Otmar Bilz; Denise Salzig; Tobias Weidner; Peter Czermak
Journal:  Biotechnol Rep (Amst)       Date:  2018-07-03
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