Literature DB >> 18265071

Purification of immunoglobulin G fraction from antiserum, ascites fluid, or hybridoma supernatant.

H M Cooper1, Y Paterson.   

Abstract

This unit describes the isolation of the immunoglobulin G (IgG) fraction (containing antibodies of all specificities) from a complex protein mixture such as antiserum, ascites fluid, or hybridoma supernatant. The basic procedure utilizes saturated ammonium sulfate solution to precipitate the IgG fraction, while an alternate protocol describes fractionation of IgG by chromatography on DEAE-Affi-Gel Blue resin.

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Year:  2001        PMID： 18265071     DOI： 10.1002/0471142727.mb1114s50

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  2 in total

1.  Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring of Trichinella spiralis Infection.

Authors:  Ning Xu; Yan Liu; Yansong Li; Bin Tang; Xiongyan Liang; Yuying Yang; Mingyuan Liu; Xiaolei Liu; Yu Zhou
Journal:  Int J Nanomedicine       Date:  2021-03-29

2.  Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204.

Authors:  Kate D Ryman; L K Metthew Lam; Alan M Watson; William B Klimstra
Journal:  NPJ Vaccines       Date:  2018-01-23       Impact factor: 7.344
  2 in total

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