| Literature DB >> 18264132 |
M Botlagunta1, F Vesuna, Y Mironchik, A Raman, A Lisok, P Winnard, S Mukadam, P Van Diest, J H Chen, P Farabaugh, A H Patel, V Raman.
Abstract
Benzo[a]pyrene diol epoxide (BPDE), the active metabolite of benzo[a]pyrene present in tobacco smoke, is a major cancer-causing compound. To evaluate the effects of BPDE on human breast epithelial cells, we exposed an immortalized human breast cell line, MCF 10A, to BPDE and characterized the gene expression pattern. Of the differential genes expressed, we found consistent activation of DDX3, a member of the DEAD box RNA helicase family. Overexpression of DDX3 in MCF 10A cells induced an epithelial-mesenchymal-like transformation, exhibited increased motility and invasive properties, and formed colonies in soft-agar assays. Besides the altered phenotype, MCF 10A-DDX3 cells repressed E-cadherin expression as demonstrated by both immunoblots and by E-cadherin promoter-reporter assays. In addition, an in vivo association of DDX3 and the E-cadherin promoter was demonstrated by chromatin immunoprecipitation assays. Collectively, these results demonstrate that the activation of DDX3 by BPDE, can promote growth, proliferation and neoplastic transformation of breast epithelial cells.Entities:
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Year: 2008 PMID： 18264132 PMCID： PMC5576029 DOI： 10.1038/onc.2008.33
Source DB: PubMed Journal: Oncogene ISSN： 0950-9232 Impact factor: 9.867