Literature DB >> 18263969

Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars.

Katarzyna Szajko1, Mirosława Chrzanowska, Danuta Strzelczyk-Zyta, Helena Zagórska, Waldemar Marczewski.   

Abstract

The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of SC811(454), which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.

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Year:  2008        PMID： 18263969     DOI： 10.1007/BF03195248

Source DB:  PubMed          Journal:  J Appl Genet        ISSN： 1234-1983            Impact factor:   3.240


  7 in total

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Journal:  Theor Appl Genet       Date:  2006-02-02       Impact factor: 5.699

5.  The Potato virus S resistance gene Ns maps to potato chromosome VIII.

Authors:  W. Marczewski; J. Hennig; C. Gebhardt
Journal:  Theor Appl Genet       Date:  2002-06-22       Impact factor: 5.699

6.  A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.

Authors:  A Konieczny; F M Ausubel
Journal:  Plant J       Date:  1993-08       Impact factor: 6.417

7.  Inter-simple sequence repeat (ISSR) markers for the Ns resistance gene in potato (Solanum tuberosum L.).

Authors:  W Marczewski
Journal:  J Appl Genet       Date:  2001       Impact factor: 3.240
  7 in total

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