Literature DB >> 18263751

Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR.

Prerak T Desai1, Marie K Walsh, Bart C Weimer.   

Abstract

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of approximately 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

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Year:  2008        PMID： 18263751      PMCID： PMC2292596          DOI： 10.1128/AEM.02601-07

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


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