OBJECTIVE: To investigate the effect of estrogen receptor beta (ERbeta) on osteoblastic differentiation function of human periodontal ligament (hPDL) cells by measuring the alkaline phosphatase (ALP) activity and the production of osteocalcin (OCN) in vitro. DESIGN: We employed a short interfering RNA (siRNA) technique to inhibit ERbeta expression in hPDL cells; the cells were cultured with a saturating concentration of 17beta-estradiol (10(-7)M). ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit. RESULTS: It was shown that estradiol significantly enhanced the ALP activity and the production of OCN in hPDL cells. However, the ALP activity and the production of OCN in hPDL-siERbeta cells were not significantly changed after estradiol treatment. CONCLUSIONS: These results indicate that ERbeta may play important roles in estrogen-induced effects on osteoblastic differentiation function of PDL cells and estrogen influences the bone formation capacity of PDL cells mainly via ERbeta.
OBJECTIVE: To investigate the effect of estrogen receptor beta (ERbeta) on osteoblastic differentiation function of human periodontal ligament (hPDL) cells by measuring the alkaline phosphatase (ALP) activity and the production of osteocalcin (OCN) in vitro. DESIGN: We employed a short interfering RNA (siRNA) technique to inhibit ERbeta expression in hPDL cells; the cells were cultured with a saturating concentration of 17beta-estradiol (10(-7)M). ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit. RESULTS: It was shown that estradiol significantly enhanced the ALP activity and the production of OCN in hPDL cells. However, the ALP activity and the production of OCN in hPDL-siERbeta cells were not significantly changed after estradiol treatment. CONCLUSIONS: These results indicate that ERbeta may play important roles in estrogen-induced effects on osteoblastic differentiation function of PDL cells and estrogen influences the bone formation capacity of PDL cells mainly via ERbeta.
Authors: Ling-Ling E; Wen-Huan Xu; Lin Feng; Yi Liu; Dong-Qing Cai; Ning Wen; Wen-Jie Zheng Journal: Int J Mol Med Date: 2016-04-12 Impact factor: 4.101