OBJECTIVES: Gallbladder carcinoma (GBC) is highly aggressive neoplasm which arises in the background of gall stones and inflammation. GBC affects women 2-3 times more frequently than men. Pro-inflammatory TNFA and IL6 gene polymorphism has been associated with various inflammatory diseases. The aim of this study was to investigate whether TNFA -308 (G/A) and IL6 -174 G/C polymorphisms within flanking region of the genes are associated with GBC susceptibility. METHODS: The promoter polymorphisms were genotyped using PCR-RFLP in 200 healthy subjects and 124 GBC patients. RESULTS: Frequency distribution of TNFA -308 (G/A) and IL6 -174 G/C were not significantly different in GBC patients in comparison to healthy controls. However, frequency of TNFA -308 (G/A) polymorphism in female GBC patients without gallstone were significantly different (p-value= 0.006) when compared to healthy female subjects (OR=3.054; 95% CI=1.39-6.72). CONCLUSION: These results suggest that TNFA -308 (G/A) polymorphism may influence the susceptibility of female gender gallbladder cancer in absence of gallstones while IL6 -174 G/C polymorphism does not seem to be playing significant role in the susceptibility to gallbladder cancer.
OBJECTIVES: Gallbladder carcinoma (GBC) is highly aggressive neoplasm which arises in the background of gall stones and inflammation. GBC affects women 2-3 times more frequently than men. Pro-inflammatory TNFA and IL6 gene polymorphism has been associated with various inflammatory diseases. The aim of this study was to investigate whether TNFA-308 (G/A) and IL6-174 G/C polymorphisms within flanking region of the genes are associated with GBC susceptibility. METHODS: The promoter polymorphisms were genotyped using PCR-RFLP in 200 healthy subjects and 124 GBC patients. RESULTS: Frequency distribution of TNFA-308 (G/A) and IL6-174 G/C were not significantly different in GBC patients in comparison to healthy controls. However, frequency of TNFA-308 (G/A) polymorphism in female GBC patients without gallstone were significantly different (p-value= 0.006) when compared to healthy female subjects (OR=3.054; 95% CI=1.39-6.72). CONCLUSION: These results suggest that TNFA-308 (G/A) polymorphism may influence the susceptibility of female gender gallbladder cancer in absence of gallstones while IL6-174 G/C polymorphism does not seem to be playing significant role in the susceptibility to gallbladder cancer.
Authors: Felipe A Castro; Jill Koshiol; Ann W Hsing; Yu-Tang Gao; Asif Rashid; Lisa W Chu; Ming-Chang Shen; Bing-Shen Wang; Tian-Qua Han; Bai-He Zhang; Shelley Niwa; Kai Yu; Hong Zhang; Stephen Chanock; Gabriella Andreotti Journal: BMC Cancer Date: 2012-10-11 Impact factor: 4.430
Authors: Ana Maria Sampaio; Sandra Caramujo Balseiro; Maria Reis Silva; Ana Alarcão; Maria João d'Aguiar; Teresa Ferreira; Lina Carvalho Journal: GE Port J Gastroenterol Date: 2015-07-04